Forward osmosis (FO) has received considerable interest for water and energy-related applications in recent years. However, FO not been commercialized yet because of a few reasons. The lack high-performance membrane is one the important barriers. To overcome this issue, novel thin-film composite (TFC) was successfully fabricated via interfacial polymerization with poly-L-lysine incorporated pol...

α-Poly(L-lysine) (α-PLL) is a water-soluble cationic biopolymer containing the monomeric unit α-L-lysine, which not only possesses excellent intrinsic properties but also can be modified via facile reactions between side amine groups and many other functional moieties. Ring-opening polymerization (ROP) most prevalently used technique for synthesis of PLL-based polypeptides, involves initial con...

Fe(II)/?-ketoglutarate-dependent dioxygenases (KDOs) catalyze a broad range of selective C–H oxidation reactions. However, the difficult production KDOs in recombinant E. coli strains and their instability purified form have so far limited application preparative biotransformations. Here, we investigated immobilization three (CaKDO, CpKDO, FjKDO) that stereoselective hydroxylation L-lysine side...

Optically active cationic complexes adsorbed on montmorillonite can be used for the resolution ofracemic mixtures. Montmorillonite-Cu-lysine systems were used as a solid phase in high-pressure liquid chromatography for the resolution of the optical isomers of a-amino acids. Selectivity constants > 1.5 were measured for phenylalanine and tryptophan. The selectivity constants for the amino acids ...

The ability to control the spatial distribution and temporal release of a therapeutic remains a central challenge for biomedical research. Here, we report the development and optimization of a novel substrate mediated therapeutic delivery system comprising of hyaluronic acid covalently functionalized liposomes (HALNPs) embedded into polyelectrolyte multilayer (PEM) platform via ionic stabilizat...

This study presents low cost fabrication of free-standing membranes in polymer with perforated pores down to subμm diameter, which provides platforms for fundamental studies of many biosystems. For the fabrication, a combination of imprint lithography and a sacrificial layer technique was employed in order to obtain a clean, fully released, and mechanically stable membrane with perforated pores...

To assay lipopolysaccharides (LPSs) in solutions containing Limulus amebocyte lysate (LAL)-inhibiting or LAL-enhancing compounds, we developed a selective endotoxin (LPS) assay using poly(epsilon-lysine)-immobilized cellulose beads (PL-Cellufine) and LAL. The PL-Cellufine can adsorb LPSs in a solution containing certain compounds (NaCl, proteins and amino acids) at an ionic strength of mu = 0.0...

We report here the development and characterization of protein microarrays fabricated on nanoengineered 3-D polyelectrolyte thin films (PET) deposited on glass slide by consecutive adsorption of polyelectrolytes via self-assembly technique. Antibodies or antigens were immobilized in the PET-coated glass slides by electrostatic adsorption and entrapment of porous structure of the 3-D polymer fil...

The thermodynamic functions such as enthalpy, H°, Gibbs free energy, G°, and entropy, S°, of Arginine and Lysine amino acids were theoretically studied at different polar solvents by using ²Gaussian o3², software. First, the structural optimization of isolated Arginine and Lysine were done in the gas phase by applying the Density Functional Theory (B3LYP) level ...

Engineered polymer films are of significant importance in the field of biomedicine. Poly(dopamine) (PDA) is becoming more and more a key player in this context. Herein, we deposited mixed films consisting of PDA and poly(L-lysine) (PLL) of different molecular weights. The coatings were characterized by quartz crystal microbalance with dissipation monitoring, atomic force microscopy, and X-ray p...