INTRODUCTION Nasal colonisation of S. aureus in healthy children was 18% to 30%. One to three percent of them were colonised by Methicillin-resistant Staphlycoccus aureus (MRSA). Although MRSA infection has become increasingly reported, population-based S. aureus and MRSA colonisation estimates are lacking. The main objective of this study was to determine the prevalence of S. aureus carriage a...

Panton-Valentine leukocidin (PVL) is a pore-forming toxin secreted by Staphylococcus aureus that has recently been associated with necrotizing pneumonia. In the present study, we report that in vitro, PVL induces polymorphonuclear cell death by necrosis or by apoptosis, depending on the PVL concentration. PVL-induced apoptosis was associated with a rapid disruption of mitochondrial homeostasis ...

Normal human immunoglobulin G (IgG) was treated with radioactive N-ethylmaleimide in the absence of a reducing agent but in the presence of a denaturing solvent. The sites of reaction were determined by isolation of the radioactive peptides from thermolytic digests ofthe heavy and light chains. Six radioactive peptides were purified from the digest of the light chain and ten from that of the he...

BACKGROUND Panton-Valentine leukocidin (PVL) is a gamma-toxin produced by Staphylococcus aureus encoded by genes lukS/lukF-PV with several single-nucleotide polymorphisms. A mutation at nucleotide position 527 results in substitution of histidine (H) to arginine (R) at amino acid 176. The groups defined based on the amino acid change, the "R isoform" group and the "H isoform" group. The purpose...

To characterize methicillin-resistant S. aureus (MRSA) isolates recovered from cystic fibrosis (CF) people during two Spanish multicenter studies (2013–2021) by whole genome sequencing (WGS). 51 MRSA were prospectively CF (51% female, median age 20 y [26–15]) 17 hospitals 2 (30 in 2013 and 21 2021). Identification was performed MALDI-TOF. WGS (Illumina-Novaseq-6000) carried out. SPAdes Prokka s...

We give a new fpt algorithm testing isomorphism of n-vertex graphs of tree width k in time 2 polylog(k) poly(n), improving the fpt algorithm due to Lokshtanov, Pilipczuk, Pilipczuk, and Saurabh (FOCS 2014), which runs in time 2 5 log k) poly(n). Based on an improved version of the isomorphism-invariant graph decomposition technique introduced by Lokshtanov et al., we prove restrictions on the s...

BACKGROUND Methicillin-resistant Staphylococcus aureus (MRSA) poses a major threat to public health worldwide. There are relatively few studies addressing the molecular epidemiology of MRSA in the Philippines. METHODS This study characterized MRSA isolates in terms of their antimicrobial susceptibility profile, the SCCmec type, and the presence of lukF-lukS genes for Panton-Valentine leukocid...

Pseudomonas aeruginosa and Staphylococcus aureus mixed-species biofilm infections are more resilient to biocide attacks compared to their single-species counterparts. Therefore, this study used an in vitro model recapitulating bacterial burdens seen in in vivo infections to investigate the interactions of P. aeruginosa and S. aureus in biofilms. RNA sequencing (RNA-seq) was utilized to identify...

OBJECTIVE To characterize a methicillin-resistant Staphylococcus aureus (MRSA) isolate responsible for an aggressive infection (peridural and psoas abscess secondary to haematogenous septic arthritis) in a poultry farmer. METHODS Molecular characterization was performed, including spa- and multilocus sequence typing of the isolate, assessment of its resistance phenotype and detection of tetra...