Luciferase exhibits a broad range of emitting frequencies. Light emission from the bioluminescence of luciferase makes it an excellent tool for monitoring gene expression, thus the control of its bioluminescence color has great bio-analytical applications. Here I use an elastic network model to examine how the sequence distribution of luciferase is related to bioluminescence multicolor emission...

Although mammalian cell factories are now widely used in production of biomedically important proteins they are still relatively ineffective compared with those from prokaryotic organisms. In an attempt to improve the CHO cell line we have used a biotechological approach to investigate whether or not the choice of signal peptide may have a major impact on the synthesis/ secretion of a recombina...

Firefly luciferase adenylates and oxidizes d-luciferin to chemically generate visible light and is widely used for biological assays and imaging. Here we show that both luciferase and luciferin can be reengineered to extend the scope of this light-emitting reaction. D-Luciferin can be replaced by synthetic luciferin analogues that increase near-infrared photon flux >10-fold over that of D-lucif...

Objective(s): siRNA may be a very promising tool for treatment of various diseases especially in cancer therapy due to high specificity. One of the main hurdles applications of siRNAs in vivo is optimization of the delivery strategy, especially the carrier systems. The aim of this study was to optimize siRNA delivery into suspended erythroleukemic cell line K562. Materials and Methods: We appli...

NanoLuc® Luciferase brings exciting new possibilities and improvements to luminescence applications, including protein stability monitoring, detection of protein interactions using BRET, and in vivo imaging. This article highlights several recent papers that illustrate the use of NanoLuc® Technology as a sensitive reporter for challenging applications beyond those of classic bioluminescence rep...

objective(s):an earlier meta-analysis on gene expression data derived from four microarray, two cdna library, and one sage experiment had identified rgs5 as one of only ten non-housekeeping genes that were reported to be expressed in human trabecular meshwork (tm) cells by all studies. rgs5 encodes regulator of g-protein signaling-5. the tm tissue is the route of aqueous fluid outflow, and is r...

The importance and applicability of ionic liquids (ILs) in biocatalysis have been well recognized. ILs have growing interest as new and highly efficient reaction mediums for biocatalytic reactions, but as a reaction milieu for firefly luciferase has not been tested. In this report, the effects of two tetramethylguanidine-based ionic liquids on the activity and stability of Photinus pyralis luci...

Schistosomes are multicellular parasites of humans exhibiting interesting biological adaptations to their parasitic lifestyle. Concerted and in depth analyses of these adaptations and their cell and molecular biology requires further development of molecular genetic tools in schistosomes. In the current study, we demonstrate that a Gaussia luciferase reporter leads to significantly higher level...

The folding of many proteins including luciferase in vivo requires the assistance of molecular chaperone proteins. To understand how a chaperone targets luciferase, we took three luciferases that give different bioluminescence with the same luciferin substrate and with differences in homology. The three luciferase genes, firefly luciferase (FF-Luc) (from Pyrocoelia miyako), and red (RE-Luc) and...

Leveraging bioorthogonal chemistry- and split-luciferase assay technologies, we have devised a new for the live-cell detection of RNA–protein interactions, RNA interaction with Protein-mediated Complementation Assay or RiPCA.