A panel of hybridomas was produced using intact Listeria monocytogenes serotype 1/2a cells as the immunogen. An IgG2a monoclonal antibody (mAb) 'mAb2B3' was isolated that reacted with L. monocytogenes but not with a representative panel of related Listeria spp. and non-Listeria spp. Binding activity was greatest against L. monocytogenes serotype 1/2a and was significantly enhanced when cells we...

introduction: this study was intended to determine the presence and distribution of listeria monocytogenes in various meat and dairy products from qazvin province by culture followed by biochemical and morphological assays. the identity of the isolates was further obtained by amplification of prfa gene in bacteria isolates. this gene is a transcriptional activator of virulence gene expression w...

background listeria monocytogenes has been isolated from various foods and environments in temperate areas, tropical countries and different parts of iran. the bacterium as a psychrotrophic organism is capable of growth at refrigeration temperatures. objective the current study was conducted to determine the incidence of listeria spp. on the surfaces of domestic refrigerators in ahvaz city as a...

DNA-based methods are increasingly important for bacterial typing. The high number of polymorphic sites present among closely related bacterial genomes is the basis for the presented method. The method identifies multilocus genomic polymorphisms in intergenic regions termed AILP (amplified intergenic locus polymorphism). For each locus, a pair of unique PCR primers was designed to amplify an in...

The genus Listeria includes (i) the opportunistic pathogens L. monocytogenes and L. ivanovii, (ii) the saprotrophs L. innocua, L. marthii, and L. welshimeri, and (iii) L. seeligeri, an apparent saprotroph that nevertheless typically contains the prfA virulence gene cluster. A novel 10-loci multilocus sequence typing scheme was developed and used to characterize 67 isolates representing six List...

RESUMEN. Presencia de Listeria monocytogenes en vegetales frescos. Introducción. En los últimos años, se ha dado un incremento en el hecho de consumir vegetales frescos como parte de una dieta sana, pero no se puede descartar el que estos pueden actuar como vehículos de microorganismos patógenos, como Listeria monocytogenes. Material y métodos. Se evaluó la presencia de Listeria monocytogenes e...

Food-associated outbreaks of human listeriosis have emphasized the importance and necessity of screening food for the presence of Listeria isolates-selective agar medium combining acriflavine (10 mg/liter) with ceftazidime (50 mg/liter) was developed. A total of 1,099 cheese production specimens were cultured, from which 157 Listeria isolates. (14.3%) grew. When compared with modified McBride a...

Bacteriocin AMA-K produced by Lactobacillus plantarum AMA-K inhibits the growth of Enterococcus spp., Escherichia coli, Klebsiella pneumoniae and Listeria spp. Growth of strain AMA-K in BHI, M17, soy milk and molasses was similar to growth in MRS. The effect of organic nitrogen sources, carbohydrates, glycerol, K2HPO4 and KH2PO4, MgSO4, MnSO4, tri-ammonium citrate, Tween 80, vitamins and initia...