We have examined the synthesis, secretion, and functional and physical characteristics of a lipid transfer protein synthesized by a human hepatocellular carcinoma line. We found that this protein shares immunochemical determinants and many other properties with the lipid transfer protein, LTP-I, which has been purified from human plasma. We conclude that the human liver cell line, HepG2, synthe...

Several proteins at endoplasmic reticulum (ER)-Golgi membrane contact sites contain a PH domain that interacts with the Golgi phosphoinositide PI(4)P, a FFAT motif that interacts with the ER protein VAP-A, and a lipid transfer domain. This architecture suggests the ability to both tether organelles and transport lipids between them. We show that in oxysterol binding protein (OSBP) these two act...

A 60-day trial with six diets (three different protein levels × two different lipid levels) containing 30, 35 and 40% protein with 10 and 15% lipid levels was conducted to determine the optimum dietary protein to lipid ratio and effects on growth performance and hematological indices of Aspikutum, Leuciscus aspius female×Rutilus frisii male. Two hundred and fifty two fish (31.2±0.4 g; mean±SEM)...

barley (hordeum vulgare l.) is a model plant, as genetic and physiological studies are concerned. abiotic stresses such as drought adversely affect the growth of plants. the objective of this experiment was to identify the responsive genes to osmotic stress, using macroarray (covering 13050 genes). rna was extracted from 5 barley genotypes in stress as well as in normal conditions at 3 times of...

OBJECTIVE SAR1b plays a significant role in the assembly, organization, and function of the coat protein complex II, a critical complex for the transport of proteins from the endoplasmic reticulum to the Golgi. Recently, mutations in SARA2 have been associated with lipid absorption disorders. However, functional studies on Sar1b-mediated lipid synthesis pathways and lipoprotein packaging have n...

Endoplasmic reticulum (ER)–plasma membrane (PM) contact sites/junctions play important roles in cell physiology including signal transduction, ion and lipid transfer, dynamics. However, little is known about the dynamic regulation functional of ER–PM junctions neurons. Using a split green fluorescent protein-based probe, we find that density sites changes dynamically dendrites hippocampal neuro...

Apolipoprotein B (apoB) and microsomal triglyceride transfer protein (MTP) are necessary for lipoprotein assembly. ApoB consists of five structural domains, betaalpha(1)-beta(1)-alpha(2)-beta(2)-alpha(3). We propose that MTP contains three structural motifs (N-terminal beta-barrel, central alpha-helix, and C-terminal lipid cavity) and three functional domains (lipid transfer, membrane associati...