Nanopores based on protein channels inserted into lipid membranes have paved the way towards a wide-range of inexpensive biosensors, especially for DNA sequencing. A key obstacle in using these biological ion channels as nanodevices is the poor stability of lipid bilayer membranes. Amphiphilic block copolymer membranes have emerged as a robust alternative to lipid membranes. While previous effo...

The chlorophyll stability in biomimetic environment is the precondition for its potential application in various fields of science and technology. Its unique role, based on the specific and very diverse chemical reactivity, justifies its use in industrial technology, pharmaceutical, food and cosmetic industry, but also in medicine. In this work, two types of liposomes were prepared from saturat...

Prooxidants are activated oxygen metabolites (AOM), active oxygen forms and free radicals of any etiology that activate free radical oxidation of all boimolecules, in particular lipids. In pathology the amount of prooxidants increases and free radical oxidation activation processes of all boimolecules, in particular lipids of biological membranes, go out of control. The accumulation of LPO prod...

The human erythrocyte sialoglycoprotein glycophorin A (GpA) has been used extensively in experiment and simulations as a model of transmembrane helix-dimer formation, emphasizing the critical role of specific residue-residue interactions between helices in dimer stability. While the tertiary dimer structure is modulated by the hydrophobic lipid bilayer environment, we show that interactions of ...

Diclofenac sodium loaded solid lipid nanoparticles (SLNs) were formulated using guggul lipid as major lipid component and analyzed for physical parameters, permeation profile, and anti-inflammatory activity. The SLNs were prepared using melt-emulsion sonication/low temperature-solidification method and characterized for physical parameters, in vitro drug release, and accelerated stability studi...

Lipid-protein complexes reconstituted from pig surfactant lipids and proteins were investigated for surface adsorption, minimum surface tension, stability index, and surface compressibility. Lipid constituents remained unchanged with procedures for the reconstitution. An apoprotein with a nominal molecular weight of 15,000 daltons significantly accelerated the lipid-protein complex to absorb th...

It was found that the exchange method for the preparation of lipid-membrane-incorporated guest molecules was applicable not only to fullerenes but also to other hydrophobic molecules such as azobenzene and stilbene. The advantages of this method are that the long-term stability of the lipid-membrane-incorporated azobenzene solution and the maximum ratio of [stilbene]/[lipid] were higher than th...

Lipid protein interactions play a key role in the stability and function of various membrane proteins. Earlier we have reported the extreme thermal stability of porin from Paracoccus denitrificans reconstituted into liposomes. Here, we used Fourier transform infrared spectroscopy for a label free analysis of the global secondary structural changes and local changes in the tyrosine microenvironm...

Solid lipid nanopaticles were developed in early 1990s as an alternative to other traditional colloidal carriers like liposomes, polymeric nanoparticles and emulsions as they have advantages like controlled drug release and targeted drug delivery with increased stability. This paper gives an overview about the potential advantages and also the disadvantages of solid lipid nanoparticles, the exc...

The thermodynamic principles underlying the structural stability of membrane proteins are difficult to obtain directly from whole proteins because of intractable problems related to insolubility in the aqueous phase and extreme stability in the membrane phase. The principles must therefore be surmised from studies of the interactions of small peptides with lipid bilayers. This review is concern...