Objective(s): Nowadays much effort is being invested in order to diagnose the mechanisms involved in neural differentiation. By clarifying this, making desired neural cells in vitro and applying them into diverse neurological disorders suffered from neural cell malfunctions could be a feasible choice. Thus, the present study assessed the capability of fetal brain extract (FBE) to induce rat bon...

in recent years transdifferentiation technology has enabled direct conversion of human fibroblasts to become a valuable, abundant and accessible cell source for patient-specific induced cell generation in biomedical research. the majority of transdifferentiation approaches rely upon viral gene delivery which due to random integration with the host genome can cause genome instability and tumorig...

objective: the organotypic co-culture is a well-known technique to examine cellular interactions and their roles in stem cell proliferation and differentiation. this study aims to evaluate the effects of dermal fibroblasts (dfs) on epidermal differentiation of adipose-derived stem cells (ascs) using a three-dimensional (3d) organotypic coculture technique. materials and methods: in this experim...

mesenchymal stem cells (mscs) have the ability to differentiate into neuronal like cells under appropriate culture condition. in this study, we investigated whether mscs derived from human peripheral blood (pb-mscs) can differentiate into neuronal like cells by synergic effect of the growth factors egf, bfgf and noggin. for this purpose, the expression of five neuronal markers (nestin, β iii tu...

Background and objectives: Modeling cancer in vivo is a very important tool to investigate cancer pathogenesis and molecular mechanisms involved in cancer progression. Laboratory mice are the most common animal used for rebuilding human cancer in vivo. Cancer stem cells (CSCs) are the main reason of failure in cancer therapy because of tumor relapse and metastasis. Isolation of cancer stem cell...

FMS-like tyrosine kinase-3 is a receptor tyrosine kinase expressed by immature hematopoietic cells and is important for the normal development of stem cells and the immune system. Mutations of FMS-like tyrosine kinase-3 have been detected in about 30% of patients with acute myelogenous leukemia and a small number of patients with acute lymphoblastic leukemia. The FMS-like tyrosine kinase-3 muta...

Background: Adipose-derived stem cells (ADSCs) are a population of pluripotent cells used for tissue engineering purposes. The main purpose of the present study was to transdifferentiate the ADSCs to Schwann-like cells and to determine the intensity of apoptosis in ADSCs during the transdifferentiation process. Methods: ADSCs were isolated from the inguinal adipose tissue of adult rats and the ...

objective: piwil2, a member of ago/piwi gene family containing piwi and paz domains, has been shown to be ectopically expressed in different cancer cells, especially its remarkable expression in cancer stem cells (cscs), and is also known to be essential for germ line stem cell self-renewal in various organisms. the hypothesis that csc may hold the key to the central problem of clinical oncolog...

Background: Bone marrow stromal stem cells (BMSC) are appropriate source of multipotent stem cells that are ideally suited for use in various cell-based therapies. It can be differentiated into neuronal-like cells under appropriate conditions. This study examined the effectiveness of co-stimulation of creatine and retinoic acid in increasing the differentiation of BMSC into GABAergic neuron-lik...