The effect of a novel immunosuppressive agent, FK506, on fresh islet allografts was evaluated in diabetic rats across major histocompatibility complex (MHC) barriers with respect to the transplantation (TR) site, islet source, treatment regimen, and antidonor antibody (Ab) titers of the recipients after TR. The functional periods of Wistar (Wi) islets transplanted under kidney capsule (KC) or i...

BACKGROUND The Lewis (LEW) and Fischer (F344) rat strains appear differentially sensitive to the aversive effects of several used and abused drugs. Naloxone, a mu opioid receptor antagonist that induces aversions in outbred rats but has no abuse potential, was assessed to determine the characteristics of compounds for which the strains differ. METHODS Opioid-naïve male LEW and F344 rats were ...

Background: It seems that there are similar associated diseases in psoriasis and Lewis (b) negative phenotypes such as diabetesmellitus, cardiovascular events, and metabolic syndrome. Therefore, we decided to evaluate the frequency of Lewis henotypes in patients with chronic plaque type psoriasis. A limited number of studies have been previously conducted in this regard.Methods: Fifty patients ...

Michael Shea and Lewis J. Kleinsmith Department of Zoology, University of Michigan, Ann Arbor, Michigan 48104 Received December 1,1972 SUMMARY Phosphorylated non-histone chromatin proteins are shown to stimulate the synthesis of RNA in a cell-free system using rat liver RNA polymerase and rat DNA as template, This stimulation is not observed when DNA's of other species are used as template, or ...

To determine whether a preoperative blood transfusion could influence corneal graft rejection, full-thickness 3 mm orthotopic corneal grafts were performed in Dark Agouti (DA) rats from syngeneic (DA) or allogeneic (Brown Norway and Lewis) inbred donors and in Lewis rats from syngeneic (Lewis) or allogeneic (DA) donors. Rats with syngeneic grafts showed no signs of rejection. The corneal button...

Experimental allergic encephalomyelitis (EAE) was induced in rats of various genotypes by injection of 10 microg guinea pig basic protein in complete Freund's adjuvant containing 100 microg H(37) RV M. tuberculosis. Histologically verified EAE was present in 20/20 Lewis, 17/17 (Lewis x BN)F(1), 9/9 Lewis backcross, and 21/42 BN backcross rats. Among the BN backcross animals, 25/42 were determin...

Reduced glomerular filtration, hypertension and renal microvascular injury are hallmarks of chronic kidney disease, which has a global prevalence of ~10%. We have shown previously that the Fischer (F344) rat has lower GFR than the Lewis rat, and is more susceptible to renal injury induced by hypertension. In the early stages this injury is limited to the pre-glomerular vasculature. We hypothesi...

Drug addiction results from the interplay between social and biological factors. Among these, genetic variables play a major role. The use of genetically related inbred rat strains that differ in their preference for drugs of abuse is one approach of great importance to explore genetic determinants. Lewis and Fischer 344 rats have been extensively studied and it has been shown that the Lewis st...

To determine whether a preoperative blood transfusion could influence corneal graft rej'ection, fullthickness 3 mm orthotopic corneal grafts were performed in Dark Agouti (DA) rats from syngeneic (DA) or allogeneic (Brown Norway and Lewis) inbred donors and in Lewis rats from syngeneic (Lewis) or allogeneic (DA) donors. Rats with syngeneic grafts showed no signs of rejection. The corneal button...

Intraperitoneal administration of group A streptococcal cell walls to Lewis rats induces a chronic arthritis, whereas the Fischer strain is resistant to the development of the lesion. Spleen cells from cell wall-treated rats (Lewis and Fischer) are deficient in the synthesis of IL-2. Using an mAb directed against the rat IL-2-R, the present studies indicate that the expression of IL-2-R on sple...