Objective. To assess the vaginal inflammatory status (VIS) in pregnant women, whether symptomatic or asymptomatic, by leukocyte quantification in relation to the microbiota during each pregnancy trimester (T). Materials and Methods. A thousand two hundred and forty eight vaginal exudates from pregnant women were prospectively examined. All the patients underwent a clinical and colposcopic exami...

BACKGROUND AND PURPOSE We tested the hypothesis that inhibition of leukocyte function by administration of monoclonal antibody 60.3 (MoAb 60.3) improves electrophysiological recovery and decreases injury volume following transient focal cerebral ischemia in cats. METHODS Halothane-anesthetized cats underwent 90 minutes of left middle cerebral artery and bilateral common carotid artery occlusi...

Henle, Gertrude (Children's Hospital of Philadelphia, Philadelphia, Pa.), and Werner Henle. Immunofluorescence in cells derived from Burkitt's lymphoma. J. Bacteriol. 91:1248-1256. 1966.-Indirect immunofluorescence tests led to the brilliant staining of a small proportion of the cells in five different cultures derived from Burkitt's (African) lymphomas. The reaction was not restricted to the 1...

Lysosomes are acidic cytoplasmic organelles that are present in all nucleated mammalian cells and are involved in a variety of cellular processes including repair of the plasma membrane, defense against pathogens, cholesterol homeostasis, bone remodeling, metabolism, apoptosis and cell signaling. Defects in lysosomal enzyme activity have been associated with a variety of neurological diseases i...

Rabbit peritoneal polymorphonuclear leukocyte lysosomes were injected into the anterior chamber. The cornea was studied in paraffin cross-sections. To study the corneal endothelium, fiat preparations were made. Nitroblue tetrazolium staining was used to study endothelial cell viability. The mitotic activity of the endothelium was estimated by tritiated thymidine incorporation. The corneas of ly...

BackgroundAppendicitis is the most common indication for an emergency operation in children's age. Although none of the laboratory values has not high sensitivity and specificity for the diagnosis of appendicitis, leukocyte count and the percentage of segmented neutrophils are most commonly used. The aim of this study was to determine whether there is a statistically significant correlation bet...

Markedly elevated111 In labeled leukocyte activity suggestive of acute osteomyelitis was observed in a region of a composite mandibular allograft that was photon deficient on 99mTc MDP bone scintigraphy. This finding was later confirmed by surgical pathology. The patient was treated with intravenous antibiotics and returned two months later with persistent bone infection. The foHow-up thr...

A healthy vascular endothelium is coated by the endothelial glycocalyx. Its main constituents are transmembrane syndecans and bound heparan sulphates. This structure maintains the physiological endothelial permeability barrier and prevents leukocyte and platelet adhesion, thereby mitigating inflammation and tissue oedema. Heparinase, a bacterial analogue to heparanase, is known to attack the gl...

The fluorescent dye ethidium bromide stains Escherichia coli and Staphylococcus aureus in whole blood. The staining is rapid, relatively specific, and does not require fixation of the sample. Furthermore, stained bacteria can be seen microscopically without the need for a final wash to remove unbound dye. By using lysostaphin, an S. aureus-specific lytic enzyme, we have demonstrated that S. aur...