Bacteriuria is the leading indicator of Urinary Tract Infection (UTI), which detected through urine culture examination. Significant bacteriuria indicates growth microorganisms as much ≥ 100,000 cfu/ml in culture. There are drawbacks to this examination that requires a screening test with short time and lower cost. This study aimed determine differences results nitrite leukocyte esterase using ...

A reusable enzyme strip for the determination of cholesterol was prepared by co-immobilizing cholesterol esterase (CEase), cholesterol oxidase (COD) and peroxidase (POD) onto alkylamine glass beads affixed on a plastic strip by a nonreactive fixative. The co-immobilized enzymes retained 71.42% of initial activity of free enzymes with a conjugation yield of 11.0 mg g beads and showed optimum act...

It is nowadays well established that a urinalysis is one of the most essential and useful diagnostic modalities in the field of urology. On many occasions, however, its key elements are neglected and omitted and the inaccurate results are produced and then misinterpreted accordingly. This occurs when the investigators collect inappropriate samples, perform the test incompletely during short per...

A new competitive immunochromatography (ICG) strip test based on polyclonal antibody (pAb) conjugated with gold nanoparticles (NPs) was developed and its applications for primary screening of immunoglobulin (Ig) A in serum were evaluated. Nanocolloidal gold as the detection reagent, with an average particle diameter of 20 nm, was synthesized and labelled pAb. The antibody-nanocolloidal gold pro...

In order to compare the performance of leukocyte esterase and nitrite urine dipstick tests with enhanced urinalysis (uncentrifuged urine white blood cell count/mm(3) plus Gram stain) in detecting asymptomatic bacteriuria in obstetric patients, clean-catch midstream urine specimens were collected from 250 consecutive asymptomatic pregnant women. Ten of the women (4.0%) showed urine culture resul...

BACKGROUND Few studies have evaluated dipstick urinalysis for elderly and practically none present confidence intervals. Furthermore, most previous studies combine all bacteria species in a "positive culture". Thus, their evaluation may be inappropriate due to Yule-Simpson's paradox. The aim of this study was to evaluate diagnostic accuracy of dipstick urinalysis for the elderly in nursing home...

There is debate about the ideal diagnostic procedure for urinary tract infections (UTIs) in general practice. The aim of this study was to evaluate nitrite and leucocyte esterase strip test procedures in general practice patients, and to relate the results to the decision of the general practitioner to prescribe antibiotic therapy. A total of 292 female patients from eight general practices in ...

Leukocyte Esterase and Hemoglobin Test Strips To the Editor: During a recent evaluation of a new glucose test (1), we also evaluated the effect of ascorbic acid on results for 1