In 4 M guanidine hydrochloride (GdnHCl), the dimeric enzyme glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides (G6PD) dissociated to subunits and was extensively unfolded. Rapid dilution of this high GdnHCl concentration allowed G6PD to partially renature, as measured by enzyme reactivation, to a level which depended on the conditions employed. The fraction of the enzyme which did...

In the routine use of typical media for the microbiological determination of the sixteen amino acids standard curves have accumulated which reveal the amounts of each amino acid required for the growth of the assay organism when all other amino acids are present in excess. Thus it was observed that the relative amounts of the various amino acids needed by Leuconostoc mesenteroides P-60 did not ...

A study on the production of soluble and cell wall-bound glucansucrases (GS) and fructansucrases (FS) by Leuconostoc mesenteroides URE13 and L. mesenteroides Lm17 during their growth on media containing sucrose, glucose or fructose as a main carbon source was carried out. The changes in the enzyme activity, the assimilation of sugars and the formation of metabolites during the cultivation were ...

14 INTRODUCTION 16 MATERIALS AND METHODS 19 Carbohydrates and reagents 19 Enzyme 19 Enzyme digest conditions 20 Digest conditions to obtain Hanes-Woolf plots 20 Digest conditions to obtain Michaelis plots 20 Determination of the amount of dextran synthesized 21 RESULTS AND DISCUSSION 22 REFERENCES 3 0 iii PAPER II INTERPRETATION OF DEXTRANSUCRASE INHIBITION AT HIGH SUCROSE CONCENTRATIONS 3 9 AB...

Shelton, Damon C. (West Virginia University Medical Center, Morgantown), and William E. Nutter. Uptake of valine and glycylvaline by Leuconostoc mesenteroides. J. Bacteriol. 88:1175-1184. 1964.-Uptake systems for valine and glycylvaline were studied in growing cultures and non-growing cellular suspensions of Leuconostoc mesenteroides. Glycylvaline promoted growth more rapidly than did valine. O...

When an unstable enzyme is incubated with its substrate(s), catalysis may cease before chemical equilibrium is attained. The residual substrate concentrations depend on their initial concentrations, the initial enzymic activity, and the inactivation rate constants for each molecular species that comprise the catalytic cycle. The underlying theory has been elaborated previously for single-substr...

The gene coding for inulosucrase in Leuconostoc citreum CW28, islA, was cloned, sequenced, and expressed in Escherichia coli. The recombinant enzyme catalyzed inulin synthesis from sucrose like the wild-type enzyme. Inulosucrase presents an unusual structure: its N-terminal region is similar to the variable region of glucosyltransferases, its catalytic domain is similar to fructosyltransferases...