The effects of digoxin on monovalent cation active transport were determined in cardiac tissue obtained from dogs given inotropic, toxic, or lethal doses of digoxin. In hemodynamically monitored dogs, active uptake of the K+ analogue Rb+ was determined in vitro in a control myocardial biopsy, and then in serial biopsies from the same dog after the infusion of [3H]digoxin in doses sufficient to ...

Phorate is an organophosphate insecticide (OPI) which is widely used throughout the world as a broad spectrum insecticide on numerous crops including paddy and groundnut. The impact of acute lethal and chronic sublethal (ALCS) toxicity of phorate was investigated on Malate dehydrogenase (MDH) activity in the vital organs of fish such as gill, liver, muscle, kidney and brain of the freshwater co...

chlorpyrifos is one of the organophosphate pesticides that are commonly used to control insects in domestic activities and many agricultural farms that are located in close to water sources. hence, the present study, investigate the lethal toxicity of this pesticide. in this study, fish were treated in groups of 21 with different concentrations of chlorpyrifos (aria brand with active ingredient...

Dihydropyrimidine dehydrogenase (DPD) enzyme deficiency is a pharmacogenetic syndrome with possible fatal outcome following 5-fluorouracil (5-FU) treatment. Several studies examining the molecular basis for DPD deficiency have identified over 30 sequence variations in the DPYD gene (which codes for the DPD enzyme). Our laboratory has recently developed and validated a denaturing high performanc...

background: iron, manganese, and aluminum are three abundant metals on earth and their concentrations have increased in aquatic environments as a result of natural and industrial activities. this study was undertaken to report the median acute toxicity (lc50) and accumulation of the sub-lethal concentration (10% 96-h lc50) of iron (fe), manganese (mn) and aluminum (al) in kutum (rutilus kutum) ...

The toxic properties of human recombinant tumor necrosis factor (TNF) were investigated in mice made hypersensitive to endotoxin by treatment with D-galactosamine. C3H/TifF mice treated with D-galactosamine were rendered sensitive to the lethal effects of submicrogram amounts of TNF. In the absence of D-galactosamine, TNF caused approximately 80% lethality with 500 micrograms. The duration of s...

Whole cells, morphological components, and various extracts of Candida albicans were tested for toxicity by methods involving biological activities ordinarily used to characterize bacterial endotoxin. Fungal preparations exerted several of these activities, but only at much higher dose levels than those required for bacterial products. Both fungal cell walls and intact cells were pyrogenic in r...

Anthrax lethal factor (LF) is a Zn2+ -metalloprotease that cleaves and inactivates mitogen-activated protein kinase kinases (MEKs). We have used site-directed mutagenesis to identify a cluster of residues in domain II of LF that lie outside the active site and are required for cellular proteolytic activity toward MEKs. Alanine substituted for Leu293, Lys294, Leu514, Asn516, or Arg491 caused a 1...

The widespread distribution of nanoplastics and nanomaterials in aquatic environments is great concern. Nanoplastics have been found to modulate the toxicity other environmental pollutants organisms, while few studies focused on their influences nanomaterials. Thus, this study evaluated polystyrene (PS) silver nanoparticles (AgNPs) zebrafish ( Danio rerio ) embryos, including acute toxicity, ox...