Maturation of a fraction of mRNAs in nematodes involves the acquisition of a common 5' terminal spliced leader sequence derived from a nonpolyadenylylated spliced leader RNA by trans splicing. We have developed a cell-free system prepared from Ascaris lumbricoides embryos that accurately and efficiently synthesized the spliced leader RNA of A. lumbricoides. Transcription of the spliced leader R...

Leader (signal) peptidases cleave the NH2-terminal leader sequences of newly synthesized secreted and membrane proteins during, or shortly after, they insert across the membrane. We have constructed a plasmid, pPS9, in which the structural gene for Escherichia coli leader peptidase is under transcriptional control of a lambda promoter, PR. pPS9 also codes for a temperature-sensitive lambda repr...

This paper considers the distributed H∞ leader-following tracking problem for a class of discretetime multi-agent systems with a high-dimensional dynamic leader. It is assumed that output information about the leader is only available to designated followers, and the dynamics of the followers are subject to perturbations. To achieve distributed H∞ leader-following tracking, a new class of contr...

AIMS To empirically validate a conceptual model of Clinical Nurse Leader integrated care delivery. BACKGROUND There is limited evidence of frontline care delivery models that consistently achieve quality patient outcomes. Clinical Nurse Leader integrated care delivery is a promising nursing model with a growing record of success. However, theoretical clarity is necessary to generate causal ev...

Mouse hepatitis virus, which replicates in cytoplasm, contains leader RNA sequences at the 5' end of the virus-specific mRNAs. We have sequenced this leader RNA by synthesizing cDNA from a synthetic oligodeoxyribonucleotide primer (15-mer) that is complementary to the sequences at the junction site between the leader and body sequences of the mRNAs. The leader sequences on each mRNA have exactl...

Leader election protocols are a fundamental building block for replicated distributed services. They ease the design of leader-based coordination protocols that tolerate failures. In partially synchronous systems, designing a leader election algorithm, that does not permit multiple leaders while the system is unstable, is a complex task. As a result many production systems use third-party distr...

We introduce the notion of stable leader election and derive several algorithms for this problem. Roughly speaking, a leader election algorithm is stable if it ensures that once a leader is elected, it remains the leader for as long as it does not crash and its links have been behaving well, irrespective of the behavior of other processes and links. In addition to being stable, our leader elect...

This paper considers the distributed H∞ leader-following tracking problem for a class of discretetime multi-agent systems with a high-dimensional dynamic leader. It is assumed that output information about the leader is only available to designated followers, and the dynamics of the followers are subject to perturbations. To achieve distributed H∞ leader-following tracking, a new class of contr...

When blood vessels are injured, leader cells emerge in the endothelium to heal the wound and restore the vasculature integrity. The characteristics of leader cells during endothelial collective migration under diverse physiological conditions, however, are poorly understood. Here we investigate the regulation and function of endothelial leader cells by plasma lithography geometric confinement g...

The plasmid gene cat-86 specifies chloramphenicol-inducible chloramphenicol acetyltransferase in Bacillus subtilis. Induction by the antibiotic is primarily due to activation of the translation of cat-86-encoded mRNA. It has been suggested that the inducer stalls ribosomes at a discrete location in the leader region of cat-86 mRNA, which causes the destabilization of a downstream RNA secondary ...