Drug resistant infectious diseases are quickly becoming a global health crisis. While Streptomyces spp. have been a major source of antibiotics over the past 50 years, efficient methods are needed to identify new antibiotics and greatly improve the rate of discovery. LCMS-based metabolomics were applied to analyze extracts of 50 Streptomyes spp. Using this methodology, we discovered bottromycin...

A new bisbenzylisoquinoline, lancifoliaine (1), together with seven known alkaloids--N-allyllaurolitsine (2), reticuline (3), actinodaphnine, norboldine, pallidine, cassythicine and boldine--were isolated from the stem bark of Litsea lancifolia (Lauraceae). In addition to that of lancifoliaine, complete ¹³C-NMR data of N-allyl-laurolitsine (2) was also reported. The alkaloidal structures were e...

BACKGROUND Accuracy of rapid diagnostic tests for dengue infection has been repeatedly estimated by comparing those tests with reference assays. We hypothesized that those estimates might be inaccurate if the accuracy of the reference assays is not perfect. Here, we investigated this using statistical modeling. METHODS/PRINCIPAL FINDINGS Data from a cohort study of 549 patients suspected of d...

The high-throughput screening and drug discovery paradigm has necessitated a change in preparation of natural product samples for screening programs. In an attempt to improve the quality of marine natural products samples for screening, several fractionation strategies were investigated. The final method used HP20SS as a solid support to effectively desalt extracts and fractionate the organic c...

This paper presents, VIANET, a new web framework addressing the distance learning scenario. The VIANET project aims at bringing together the most promising web technologies and standards, in order to attain a highly competitive online learning environment. Moreover, the new web framework includes a Learning Management System (LMS), a SCORM based Learning Content Management System (LCMS) and a V...

In this work is proposed that an adaptation tool that is for the automatic generation and personalization of courses of a general-purpose LCMS that is named A Tutor. A Tutor is a Learning Content Management System. The architecture of the adaptation tool that allows a personalized sequencing of LOs in A Tutor for the learner’s learning goals, learning styles and cognitive state is presented.

We present the use of hydrazone dynamic combinatorial libraries (DCLs) to identify macrocyclic receptors that are selective for alkaline earth metal ions over alkali metal ions. In particular, the toxic heavy metal ions Sr(2+) and Ba(2+) induce characteristic changes in the DCLs. Four macrocycles were isolated and characterised by LCMS, HRMS, NMR and X-ray crystallography; binding studies by UV...

Fractionation of petroleum ether partitioned ethanol extract and crude petroleum ether extract of the leaves of Murraya koenigii Linn (Rutaceae) led to the isolation of 5,8-dimethyl furanocoumarin (1) and 1-al, 3[6’, 6’ dimethyl 5-hexene] carbazole (2) and β-sitosterol (3). Their structures were elucidated by spectroscopic methods such as UV, IR, NMR and LCMS. All the compounds were isolated fo...