A semi-quantitative latex agglutination test for bovine serum CRP levels has been established by mixing diluted serum (or diluted standard serum) with a 1% latex suspension containing 0.489 micron latex particles coated with affinity-purified antibody at a ratio of 20 micrograms/mg latex. The agglutination was performed on a glass slide in a moist chamber at room temperature with 45 min. incuba...

A commercially available latex agglutination test for Clostridium difficile was compared with a cell culture cytotoxin assay and bacteriological culture for the laboratory diagnosis of C. difficile-associated diarrhea and colitis (CAD). Stool specimens from 626 patients were tested by the three methods, and specimens from 118 patients (19%) were positive by at least one of the methods. The resu...

A rapid reversed passive latex agglutination method that uses high-density latex particles for the detection of staphylococcal enterotoxins (SE) A to E was developed. It took 3 h for incubation, much less than the 16 h needed with a customary latex agglutination test for SE detection such as a commercial test kit (SET-RPLA; Denka Seiken Co. Ltd., Tokyo, Japan). The rapid test was shown to be hi...

A semi-quantitative latex agglutination test for bovine serum CRP levels has been established by mixing diluted serum (or diluted standard serum) with a 1% latex suspension containing 0.489 #m latex particles coated with affinity-purified antibody at a ratio of 20 #g/mg latex. The agglutination was performed on a glass slide in a moist chamber at room temperature with 45 min. incubation. This t...

Current techniques for rapid diagnosis of microbial infections by direct detection of the microbial agent are compared. The techniques include enzyme immunoassay (EIA) tests, immunofluorescence, latex agglutination assays, and nucleic acid hybridization procedures. It is concluded that, for the near future, the preferred methods for rapid diagnosis will be by (1) EIA tests utilizing monoclonal ...

Soluble antigens of Haemophilus influenzae type b, Streptococcus pneumoniae, Neisseria meningitidis, and group B streptococcus were looked for in cerebrospinal fluid, serum, and urine by using the staphylococcal coagglutination test, latex agglutination test, and counterimmunoelectrophoresis. The staphylococcal coaggultination and latex agglutination tests were more sensitive than counterimmuno...

In mice infected with an avirulent strain of Toxoplasma gondii, circulating Toxoplasma antigens were detectable in the sera during weeks 1 to 3 of infection by a simple agglutination test that uses latex particles coated with anti-Toxoplasma antibodies. An infection in athymic nude mice resulted in high agglutination titers in the anti-Toxoplasma antibody-coated latex particle test and the abse...