Aims: To evaluate whether orocoecal transit time (OCTT) might improve the correct allocation (lactose absorbers vs malabsorbers) of subjects with “borderline” H2 Breath test (H2BT) values ranging 10-20 ppm, and to determine among malabsorbers if OCTT can aid to discern lactose intolerant from tolerant individuals. Patients and Methods: OCTT and increment of H2 levels in breath following a dose ...

Polyethylene glycol-treated protoplasts prepared from Streptococcus lactis LM3302, a lactose-negative (Lac) derivative of S. lactis ML3, were transformed to lactose-fermenting ability by a transductionally shortened plasmid (pLM2103) coding for lactose utilization.

Background: The prevalence of genetically determined lactase non-persistence is based on the results of the lactose H 2 breath test. This test, however, is an indirect test, which might lead to misinterpretation. Design: We determined lactase activity in healthy Chinese and Dutch students using a novel C-lactose digestion test. The cut-off value of this test was established in a Chinese populat...

This application note reports on the development of a calibration model, its validation and applicability for the determination of residual lactose in lactose-reduced milk and milk products using a Lactoscope FTIR Advanced (FTA) Instrument. The calibration model developed for milk was based on a set of 76 samples, including process samples, endproducts of various brands of skim milk, semi skim ...

Agrobacterium radiobacter NCIB 11883 was grown in lactose-limited continuous culture at a dilution rate of 0.045/h. Washed cells transported [14C]lactose and [methyl-14C]beta-D-thiogalactoside, a nonmetabolisable analog of lactose, at similar rates and with similar affinities (Km for transport, less than 1 microM). Transport was inhibited to various extents by the uncoupling agent carbonyl cyan...

The heterodisaccharide lactose (1,4-O-beta-D-galactopyranosyl-D-glucose) induces cellulase formation in the ascomycete Hypocrea jecorina (= Trichoderma reesei). Lactose assimilation is slow, and the assimilation of its beta-D-galactose moiety depends mainly on the operation of a recently described reductive pathway and depends less on the Leloir pathway, which accepts only alpha-D-galactose. We...

Lactulose is a synthetic disaccharide which has remarkable applications in food and pharmaceutical fields. Lactulose is not found in nature and it is produced by isomerization reaction of lactose in an alkaline environment. It should be noted that this reaction has a very low yield since significant amount of lactose stays unreacted in the system. Basically, purification of lactulose is difficu...

Functional food for lactose-intolerant consumers and its global prevalence has created a large market for commercially available lactose-free food products. The simplest approach for detection and quantitation of lactose in lactose-free dairy products was developed. A one-step sample preparation was employed and the resulting 10% sample solution was directly subjected to the chromatographic sys...

Galectins are β-galactoside-binding proteins implicated in a myriad of biological functions. Despite their highly conserved carbohydrate binding motifs with essentially identical structures, their affinities for lactose, a common galectin inhibitor, vary significantly. Here, we aimed to examine the molecular basis of differential lactose affinities amongst galectins using solution-based techniq...

Using breath hydrogen analysis after 139 mmol (50 g) oral lactose load, we investigated the prevalence of lactose malabsorption in 200 Greek adults and examined the relationship between symptoms and small bowel transit time. One hundred and fifty subjects had increased breath hydrogen concentrations (greater than 20 ppm) after the lactose load. In these individuals peak breath hydrogen concentr...