Changes in metabolic enzyme activities were examined in three species of pikas that occur over a range of altitudes. Because these closely related mammals live in comparable ecosystems and face similar environmental factors regardless of altitude, modifications of metabolic machinery are probably due to differences in oxygen availability. Citrate synthase (CS), beta-hydroxyacyl CoA dehydrogenas...

Raised plasma lactate dehydrogenase (LDH) values were found in 26 patients with marked megaloblastic anaemia due either to vitamin B(12) or folic-acid deficiency or a combination of these factors.Minor megaloblastic changes were not usually accompanied by plasma LDH elevation. Serial LDH estimations were as valuable as serial reticulocyte counts in assessing the response to physiological doses ...

This study systematically investigated how anionic surfactants of various hydrophilicities affected the activities of three metabolically important enzymes –– glutamate dehydrogenase (GDH), lactate dehydrogenase (LDH), and malate dehydrogenase (MDH) –– of different molecular masses at a pH range important to body functions (6.5-7.4). We also investigated the time course of the surfactant concen...

In a previous study, an Escherichia coli strain lacking the key enzymes (acetate kinase and phosphotransacetylase, ACK-PTA) of the major acetate synthesis pathways reduced acetate accumulation. The ackA-pta mutant strain also exhibits an increased lactate synthesis rate. Metabolic flux analysis suggested that the majority of excessive carbon flux was redirected through the lactate formation pat...

Little is known about the effect of chronic hypobaric hypoxia on the enzymes and transporters involved in lactate metabolism. We looked at the protein expression of monocarboxylate transporters MCT 1, MCT 2, and MCT 4, along with total lactate dehydrogenase (LDH) and LDH isozymes in skeletal muscle, cardiac muscle, and liver. Expression of these components of the lactate shuttle affects the abi...

the aim of this study was to assess the effects of intermittent training on lactate level and lactate dehydrogenase enzyme activity in wistar rats. 20 male wistar rats (mean age 3 months and weight 224±14 g) were selected and randomly divided into the training (n=10) and control (n=10) groups. the training protocol consisted of running on a treadmill for 4 minutes and then 2 minutes of active r...

Lactate, the conjugate base of lactic acid occurring in aqueous biological fluids, has been derided as a "dead-end" waste product of anaerobic metabolism. Catalyzed by the near-equilibrium enzyme lactate dehydrogenase (LDH), the reduction of pyruvate to lactate is thought to serve to regenerate the NAD(+) necessary for continued glycolytic flux. Reaction kinetics for LDH imply that lactate oxid...

Purpose: To investigate the relationship between lactate dehydrogenase A (LDH-A) expression, lactate concentration, cell metabolism, and metastases in murine 4T1 breast tumors. Experimental Design: Inhibition of LDH-A expression and protein levels were achieved in a metastatic breast cancer cell line (4T1) using short hairpin RNA (shRNA) technology. The relationship between tumor LDH-A protein ...

Background: Breast cancer is the most common cancer in women. Worldwide, it is estimated that more than 1 million women are diagnosed with breast cancer every year. Cancer of the breast is so widespread that it has become a genuine problem for public health. Aim: To analyse serum Lactate Dehydrogenase levels (preoperatively and postoperatively) and investigate the lipid profile in female breast...

Glucose is a main metabolic substrate in the central nervous system (CNS). Recently, lactate has attracted renewed attention because it plays an important role in the CNS as a metabolic substrate between neurons and astrocytes. Lactate and lactate dehydrogenase (LDH), a key enzyme for lactate and pyruvate interconversion, have been investigated. The glycogen body (GB) is a gelatinous tissue in ...