The effect of hemolysis on several assays performed with the Hitachi 717 was quantified by relating the amount of error to the concentration of hemoglobin. Hemolysis interference was judged clinically significant when analyte concentration varied by > 10% from the initial value. Hemolysis interference was significant for alkaline phosphatase, aspartate aminotransferase, alpha-amylase, bilirubin...

Two new Cu(II)-based coordination polymers, one of which is the {[Cu(?3-fdc)(?-dptztz)(H2O)]·DMF}n (1) and another {[Cu(?3-tdc)(?-dptztz)]·DMF}n (2) (fdc = 2,5-furandicarboxylate, dptztz 2,5-di(4-pyridyl)thiazolo[5,4-d]thiazole tdc 2,5-thiophenedicarboxylate,) were synthesized by Cu(NO3)2·3H2O reacting with different carboxylate ligands (H2fdc H2tdc for 1 2, respectively). Single-crystal X-ray ...

The changes in conformation of F-actin induced by the binding of the glycolytic enzyme lactate dehydrogenase were studied in myosin-free single ghost muscle fibres. The formation of the lactate dehydrogenase-F-actin complex was accompanied by changes in the parameters of intrinsic (tryptophan) and extrinsic (rhodaminyl-phalloin) polarized fluorescence of ghost muscle fibre F-actin. Lactate dehy...

A lactate dehydrogenase activity is present in rice (Oryza sativa L.) seedlings and roots. Under aerobic conditions, lactate dehydrogenase activity is barely detectable in rice seedlings and is very low in rice roots. In 30 day old roots, the activity is increased two to three times by an anoxic or hypoxic treatment and can be detected on immunoblots by an antiserum raised against barley lactat...

Leuconostoc mesenteroides increased its lactic acid production from glucose threefold when malic acid was added to the culture. This increase resulted also in a reduction of the ratio of d-lactic acid to l-lactic acid (31.5 to 1.23). Addition of malic acid increased 6.5-fold the specific activity of nicotinamide adenine dinucleotide (NAD)-linked l-lactate dehydrogenase and increased 3.2-fold th...

OBJECTIVE Altered mental status is a common cause of intensive care unit admission in children. Differentiating structural causes of altered mental status from metabolic etiologies is of utmost importance in diagnostic approach and management of the patients. Among many biomarkers proposed to help stratifying patients with altered mental status, spinal fluid lactate dehydrogenase appears to be ...

In the preceding paper the preparation and characterization of antiserum to purified D-lactate are described. In this paper the effects of the antibody on D-lactate dehydrogenase activity and D-lactate-dependent active transport in native Escherichia coli ML 308-225 membrane vesicles and ML 308-225dld-3 vesicles reconstituted with D-lactate dehydrogenase are described. The results demonstrate t...

Kinetic methods are described for determination of total, heat-stable, and urea-stable lactate dehydrogenase (EC 1.1.1.27) activity on the centrifugal analyzer. In the urea-stable method we present, 2.6 molar urea is used to differentiate lactate dehydrogenase originatingfrom heart and liver.This urea concentration, greater than that used by most other investigators, better differentiates betwe...