The occurrence of resistances in Gram negative bacteria is steadily increasing to reach extremely worrying levels and one the main causes resistance massive spread very efficient ?-lactamases which render most ?-lactam antibiotics useless. Herein, we report development a series imino-analogues ?-lactams (namely azetidinimines) as non-covalent inhibitors ?-lactamases. Despite structural mechanis...

OBJECTIVES Outbreaks of OXA-48-like carbapenemase producers are increasingly reported in many European countries and are often the result of difficulties in detection, especially for isolates with MICs of carbapenems that remain in the susceptibility range. METHODS An in-house real-time quantitative PCR (qPCR) assay using TaqMan chemistry to detect bla(OXA-48-like) genes was compared with bac...

The antimicrobial susceptibility profiles of Salmonella spp. isolated from livestock production systems in Sicily were determined. antibiotic sensitivity and broad-spectrum beta-lactamase strains assessed by detecting β-lactamases blaCTX-M IV, TEM, OXA SHV, blaCMY II, CTX-M I, DHA. In total, 93.3% showed multi-drug resistance (MDR). A total seven serotypes (i.e., Infantis, S. Typhimurium (monop...

A collection of 15 carbapenem-resistance Acinetobacter baumannii clinical isolates was analysed on two tertiary hospitals in Mexico. The OXA-51 was identified in all isolates, followed by OXA-239 and OXA-58; OXA-239 is described as a new OXA-23-like allele. These carbapenemases were identified on four clonal groups, distributed between two neighbouring hospitals. Acinetobacter baumannii is poor...

Enterobacteriaceae producing class D OXA-48 and OXA-48-like carbapenemases are an increasing concern. Detection is a diagnostic challenge because (i) some isolates are carbapenem susceptible and may go unrecognized, (ii) tests that detect carbapenem hydrolysis may be insensitive due to the relatively weak hydrolytic activity of these enzymes, (iii) the enzymes are not significantly inhibited by...

The current phenotypic methods for detecting carbapenemase-producing Enterobacteriaceae (CPE) allow differentiation between class A and B carbapenemases, but they cannot confirm in a single test class D OXA-48 carbapenemase producers. In this study, we evaluated a new phenotypic test, the OXA-48 disk test, which is based on an imipenem disk and two blank disks adjacent to the imipenem disk, loa...

BACKGROUND AND OBJECTIVE Carbapenems are therapeutic choice against infections caused by gram-negative bacilli including strains of Acinetobacter baumannii. Resistance to these antibiotics is mediated by efflux pumps, porins, PBPs and ß-lactamases. The aim of this study was to determine the possibility of existence of MBLs, OXAs and GES-1 betalactamase genes among clinical isolates of Acinetoba...

Carbapenem resistance in Acinetobacter spp. is an emerging problem in China. We investigated the molecular epidemiology and carbapenemase genes of 221 nonrepetitive imipenem-resistant clinical isolates of Acinetobacter spp. collected from 1999 to 2005 at 11 teaching hospitals in China. Genotyping by pulsed-field gel electrophoresis (PFGE) found 15 PFGE patterns. Of these, one (clone P) was iden...