The pH stability of the free laccase and the immobilized laccase was determined by incubating the enzyme in different buffers (pH 3.0-8.0) for 6 h at 35 °C. The buffers used were Na2HPO4–sodium citrate buffer (pH 3.0-8.0). The immobilized laccase was collected by centrifugation (3500 g for 3 min) and air-dried. The residual specific activities of free or immobilized laccase were then determined...

The construction of optimized biological fuel cells requires a cathode which combines the longevity microbial catalyst with current density an enzymatic catalyst. Laccase-secreting fungi were grown directly on cell to facilitate exchange inactive enzymes active enzymes, goal extending lifetime laccase cathodes. Directly incorporating laccase-producing fungus at extends operational cathodes whil...

A bacteria strain Bacillus thuringiensis RUN1identifiedby 16s RNA gene phylogenetic analysiswas used to decolorize malachite green, a triphenylmethane dye in simulated wastewater. The ability of the organism produce extracellular laccase and degrade were also investigated. Results showed that decolorized (84.67 ± 1.19 %) green at 40 mgl-1 within 6 h; decolorization was associated production by ...

Laccase from Trametes hirsuta basidiomycete has been covalently bound to graphite electrodes electrochemically modified with phenyl derivatives as a way to attach the enzyme molecules with an adequate orientation for direct electron transfer (DET). Current densities up to 0.5mA/cm(2) of electrocatalytic reduction of O(2) to H(2)O were obtained in absence of redox mediators, suggesting preferent...

To achieve effective decolorization of reactive dyes, laccase immobilization was investigated. Laccase 0.2% (m/V) (Denilite IIS) was trapped in beads of alginate/gelatin blent with polyethylene glycol (PEG), and then the supporters were activated by cross-linking with glutaraldehyde. The results of repeated batch decolorization showed that gelatin and appropriate concentration of glutaraldehyde...

A novel magnetically separable laccase immobilized system was constructed by adsorbing laccase into bimodal carbon-based mesoporous magnetic composites (CMMC). A large adsorption capacity (491.7 mg g(-1)), excellent activity recovery (91.0%) and broader pH and temperature profiles than free laccase have been exhibited by the immobilized laccase. Thermal stability was enhanced to a great extent ...

Laccases are multicopper oxidoreductases that can be used in biofuel cells to improve cathode performance by cathodic oxygen reduction. Here we present a laccase from the ligninolytic white-rot fungus Pycnoporus sanguineus that, in contrast to the Trametes versicolor laccase, can be produced in the absence of inducers in a standard culture medium. After 7days of cultivation the activity of this...

A double-stranded RNA virus of the chestnut blight pathogen, Cryphonectria parasitica, has been shown previously to reduce accumulation of mRNAs of extracellular laccase (laccase A) produced by this fungus. Both extra- and intracellular laccases have been detected after growth of the fungus in liquid culture. In addition to cellular localization, the two laccases are distinguishable by time of ...