BACKGROUND Uraemia and dialysis are viewed as catabolic processes resulting in malnutrition in chronic renal failure (CRF) patients. To sort out the effects of uraemia, acidosis, and dialysis on protein metabolism, we measured leucine flux in CRF patients before and after initiation of maintenance dialysis. SUBJECTS AND METHODS Whole-body leucine flux was measured by primed-constant infusion ...

Lack of insulin results in a catabolic state in subjects with insulin-dependent diabetes mellitus which is reversed by insulin treatment. Amino acid supply, especially branched chain amino acids such as leucine, enhances protein synthesis in both animal and human studies. This small study was undertaken to assess the acute effect of supplemental leucine on protein metabolism in adolescents with...

Leptin is an adipocyte-derived hormone participating in the regulation of food intake and energy balance. Its secretion from fat cells is potentiated by insulin and by substrates providing ATP, whereas factors increasing cAMP level attenuate hormone release stimulated by insulin and glucose. The present experiments were aimed to determine the effect of cAMP on leptin secretion stimulated by glu...

In this study, we demonstrated the asymmetric synthesis of L-tert-leucine from trimethylpyruvate using branchedchain aminotransferase (BCAT) from Escherichia coli in the presence of L-glutamate as an amino donor. Since BCAT was severely inhibited by 2-ketoglutarate, in order to overcome this here, we developed a BCAT/aspartate aminotransferase (AspAT) and BCAT/AspAT/pyruvate decarboxylase (PDC)...

Enantioanalysis of amino acids became a key factor in the metabolomics cancer. As screening method, it can provide information about state health patients. The main purpose study is to develop highly reliable enantioanalysis method for determination D-, and L-leucine biological samples order establish their role as biomarkers diagnosis breast Two enantioselective stochastic sensors based on N-m...

The biosynthesis of lactacystin, a new microbial metabolite which induces differentiation of neuroblastoma cells, was studied by the feeding experiments of various 13C-labeled compounds and NMR spectroscopic analysis. The feeding experiments showed that lactacystin consists of three containing gamma-lactam moiety arises by a condensation between methylmalonic semialdehyde and C alpha position o...

Bergmann and Stein (1) have described a procedure for the isolation and purification of naturally occurring n-leucine based upon the precipitation and recrystallization of the slightly soluble salt of this amino acid with naphthalene-fi-sulfonic acid. The product obtained was stated to be 97 per cent pure and to be free from methionine, a common contaminant of less pure preparations of n-leucin...