نتایج جستجو برای: km and vmax
تعداد نتایج: 16830427 فیلتر نتایج به سال:
Functional protein-protein interactions between UDP-glucuronosyltransferase (UGT)1A isoforms and cytochrome P450 (CYP)3A4 were studied. To this end, UGT1A-catalyzed glucuronidation was assayed in Sf-9 cells that simultaneously expressed UGT and CYP3A4. In the kinetics of UGT1A6-catalyzed glucuronidation of serotonin, both Michaelis constant (Km) and maximal velocity (Vmax) were increased by CYP...
Phospholipid nanogels enhance the stability and performance of the exoglycosidase enzyme neuraminidase and are used to create a fixed zone of enzyme within a capillary. With nanogels, there is no need to covalently immobilize the enzyme, as it is physically constrained. This enables rapid quantification of Michaelis-Menten constants (KM) for different substrates and ultimately provides a means ...
The kinetics of RNase H promoted cleavage of the RNA strand in the antisense oligonucleotide (AON)–RNA hybrid duplexes, with the 3 -end of the AON strand tethered with cholic acid (2), its triacetate (3), cholesterol (4) or dipyridophenazine (5), have been investigated by changing the concentration of both the AON and the RNA strands, while keeping the enzyme and the buffer concentration consta...
We have examined the interaction of hepatic phenylalanine hydroxylase with the phenylalanine analogs, tryptophan and the diastereomers of 3-phenylserine (beta-hydroxyphenylalanine). Both isomers of phenylserine are substrates for native phenylalanine hydroxylase at pH 6.8 and 25 degrees C, when activity is measured with the use of the dihydropteridine reductase assay coupled with NADH in the pr...
The concentration of urea in renal medullary cells is high enough to affect enzymes seriously by reducing Vmax or raising Km, yet the cells survive and function. The usual explanation is that the methylamines found in the renal medulla, namely glycerophosphocholine and betaine, have actions opposite to those of urea and thus counteract its effects. However, urea and methylamines have the simila...
We apply the Jeans equation to estimate masses for eight of the brightest dwarf spheroidal (dSph) galaxies. For Fornax, the dSph with the largest kinematic data set, we obtain a model-independent constraint on the maximum-circular velocity, Vmax = 20 +4 −3 km s . Although we obtain only lowerlimits of Vmax & 10 km s −1 for the remaining dSphs, we find that in all cases the enclosed mass at the ...
آنزیم پراکسیداز (EC 1.11.1.7) جزء پروتئین‌های هم‌دار می‌باشد که وظیفه اصلی آنها اکسیداسیون سوبستراهای مختلف با استفاده از پراکسید هیدروژن می‌باشد. پراکسیداز کاربرد گسترده‌ای در تشخیص‌های پزشکی و تجربیات آزمایشگاهی مختلف دارد، به همین علت امروزه تلاش زیادی صورت می‌گیرد تا منابع جدیدی از بافت‌های گیاهی با فعالیت پراکسیدازی مناسب معرفی گردد. کدو سبز (cucurbita pepo) نوعی کدوی تابستانی است که می‌تو...
Background: Phospholipases are one of the numerous enzymes found in Naja mossambica venom. They play a major role snakebite envenomation, and also responsible for hydrolysis phospholipid, disrupting membrane integrity. In this study, we evaluated effect Vernonia amygdalina on Phospholipase activity from (Cobra) Results: Partially purified phospholipase had maximal velocity (Vmax) Michaelis Ment...
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