A preparation of two commercial enzymes was used to liberate protoplasts from 16-h-old mycelium of Phanerochaete chrysosporium. Regeneration frequencies of up to 5% were attained when the protoplasts were plated in a medium containing 10% sorbose and 3% agar. Fusion of protoplasts from different auxotrophic strains in polyethylene glycol-Ca produced heterokaryons. Separation of the heterokaryon...

Benzotriazole and methyl benzotriazole were studied in soil, water, and wood. As reported by various authors, benzotriazole is highly stable in soil or water. It was also found to be a potent inhibitor of plant growth, killing several species at levels of about 0.1 mg/ml. Both benzotriazoles are readily degraded by the Fenton reaction; they are also degraded slowly in the presence of horseradis...

A high degree of lipid oxidative damage was detected in manganese-deficient lignin peroxidase (LIP)-producing cultures of Phanerochaete chrysosporium, indicating intensive exposure to high concentrations of reactive oxygen species (ROS), as is typically found in oxygenated LIPproducing cultures. The absence of manganese ions (Mn2+) in the cultures prevented activation of the manganese-containin...

The ability of Phanerochaete laevis HHB-1625 to transform polycyclic aromatic hydrocarbons (PAHs) in liquid culture was studied in relation to its complement of extracellular ligninolytic enzymes. In nitrogen-limited liquid medium, P. laevis produced high levels of manganese peroxidase (MnP). MnP activity was strongly regulated by the amount of Mn2+ in the culture medium, as has been previously...

Two families of peroxidases-lignin peroxidase (LiP) and manganese-dependent lignin peroxidase (MnP)-are formed by the lignin-degrading white rot basidiomycete Phanerochaete chrysosporium and other white rot fungi. Isoenzymes of these enzyme families carry out reactions important to the biodegradation of lignin. This research investigated the regulation of LiP and MnP production by Mn(II). In li...

Under nitrogen-limiting, secondary metabolic conditions, the white rot basidiomycete Phanerochaete chrysosporium extensively mineralized the specifically 14C-ring-labeled azo dyes 4-phenylazophenol, 4-phenylazo-2-methoxyphenol, Disperse Yellow 3 [2-(4'-acetamidophenylazo)-4-methylphenol], 4-phenylazoaniline, N,N-dimethyl-4-phenylazoaniline, Disperse Orange 3 [4-(4'-nitrophenylazo)-aniline], and...