Soybean lipoxygenase-1 (SLO-1) is a paradigmatic enzyme system for studying the contribution of hydrogen tunneling to enzymatic proton-coupled electron transfer processes. In this study, the impact of pairs of double mutants on the properties of SLO-1 is presented. Steady-state rates and their deuterium kinetic isotope effects (KIEs) have been measured for the bimolecular reaction of enzyme wit...

The structural stability of a peroxidase, a dimeric protein from palm tree Chamaerops excelsa leaves (CEP), has been characterized by high-sensitivity differential scanning calorimetry, circular dichroism and steady-state tryptophan fluorescence at pH 3. The thermally induced denaturation of CEP at this pH value is irreversible and strongly dependent upon the scan rate, suggesting that this pro...

This thesis has investigated oxygen uptake ( i r02) kinetic regulation during whole body exercise by investigating not only the breath-by-breath V O ~ response to a change in work rate but also incorporating an examination of data analysis processes and simulation of a physiological V O ~ model. Five experiments have been completed to answer the following questions: Experiment 1: What effects d...

The prediction of protein-protein kinetic rate constants provides a fundamental test of our understanding of molecular recognition, and will play an important role in the modeling of complex biological systems. In this paper, a feature selection and regression algorithm is applied to mine a large set of molecular descriptors and construct simple models for association and dissociation rate cons...

The adsorption of methyl tert-butyl ether by granular activated carbon was investigated. The experimental data were analyzed using the Freundlich isotherm and the Langmuir isotherm. Although equilibrium data were found to follow Freundlich isotherm model, it were fitted better by the Langmuir model with a maximum adsorption capacity of 204.1 mg/g. The kinetic data obtained at different concentr...

reactions of free radicals and reactive oxygen species (ros) with biological molecules in vivo play an important physiological role in many diseases. 2,2-diphenyl-1-picrylhydrazyl (dpph) is a stable free radical and often used as a routine reagent to evaluate the antioxidant capacity of an antioxidant. this study was undertaken to investigate the free radical-scavenging and antioxidant activiti...

the kinetics and mechanism of the reaction of 5-nitro-1h-benzo[d] imidazole to produce 6-nitro-1h-benzo[d] imidazole was studied by employing hybrid meta-density functional theory. mpwblk/6-31+g** level calculations were carried out to obtain energies and optimize the geometries of all stationary points along the pes, and determine the harmonic vibrational frequencies. two transition states (ts...

Until now, all methods for temperature sensing in capillary electrophoresis (CE) relied on molecular probes with temperature-dependent spectral/optical properties. Here we introduce a nonspectroscopic approach to determining temperature in CE. It is based on measuring a temperature-dependent rate constant of complex dissociation by means of a kinetic CE method known as nonequilibrium capillary ...

In this study, kinetics of biological carbon, nitrogen, and phosphorous removal from a synthetic wastewater in an integrated rotating biological contactoractivated sludge system was investigated. The experimental data obtained from varying four significant independent factors viz., hydraulic retention time, chemical oxygen demand for nitrogen to phosphorus ratio, internal recirculation from aer...

AbstractThe purpose of this study was to investigate the controllable operating parameters influence, including pH, solid content, collector, co-collector, and depressant dose, and conditioning time, on apatite flotation kinetics. Four first order flotation kinetic models are tested on batch flotation time-recovery profiles. The results of batch flotation tests and the fitting of first-order ki...