Keto-bromothymol blue [BTB]keto was quantitatively prepared by potassium permanganate oxidation of bromothymol in an alkaline medium at pH > 12. When reacting with dinitrophenyl hydrazine and hydroxylamine, the obtained keto derivatives were characterized FTIR, TDDFT-DFDT computations, optical properties. The resulting compounds found to have a high chelation affinity most hydroxylamine forming...

• Pillar array columns was applied to α-keto acids analysis. α-Keto were derivatized with 1,2-diamino-4,5-methylenedioxybenzene for fluorescence detection. Four separated on pillar in 160 s. Three branched-chain human plasma samples successfully quantified. Branched-chain (BCKAs, namely, α-ketoisovaleric acid (KIV), α-ketoisocaproic (KIC), and α-keto-β-methylvaleric (KMV)) are related many dise...