In vitro culture of primary hepatocytes for drug screening purposes remains a challenge as cells rapidly lose their function in conventional conditions. Thin oxygen permeable membranes have shown, through direct oxygenation, beneficial effects long-term and cellular with freshly isolated hepatocytes. However, cryopreserved hepatocytes, standard the industry, has shown limits due to high damage,...

Objective – Our study aimed at evaluating changes in liver tissues after simulated injury and use of novel hemostatic agents (containing tranexamic acid) the course vivo experiment. Material Methods The following were studies: TachoComb collagen plate (Group 1); samples local based on sodium carboxymethyl cellulose (CMC), Na-CMC 2); + acid 3). We performed 30 rabbits with laceration via laparos...

Milk thistle (Silybum marianum) is a native plant of Mediterranean Sea areas. Silymarin, an extract from its seeds, is used in various liver diseases for its hepatoprotective effects. It contains silymarin flavonoids (SF) such as silybin, silydianin and silychristin. Cyclosporin A (CsA) is widely used as an immunosuppressant in organ transplantations. Its other applications in many autoimmune d...

The aim of this study was to investigate the possible effects maternal administration lead acetate on morphological structure liver; AST, ALT, ALP and GGT levels glycogen content hepatocytes male offspring wistar rats. Female rats were exposed 0.2% in drinking water performed their (7 groups). To evaluate liver enzymes, blood samples taken from after anesthesia. After dissection, its hepatocyte...

The effects and interaction between cAMP-analogue dibutyryl-cAMP and calmodulin antagonists were investigated on de novo synthesis and secretion of lipids in cultures of hepatoma McArdle-RH7777 cells and normal rat hepatocytes. Dibutyryl cAMP caused a significant decrease in the secretion of de novo synthesized triacyl [3H] glycerol in both cultures of McArdle cells and rat hepatocytes. The ...