Effect of various fermentation media, carbon sources, nitrogen sources, phosphate concentration and culture requirements includes inoculum levels and age were determined on gentamicin production and biomass dry weight production for Micromonospora echinospora, a gentamicin producing strain. Of the substrates tested, starch as a sole carbon source promoted maximal gentamicin production, while ma...

The aim of this study was to enhance the production of vitamin K2 by using N-methyl-N-nitro-N-nitroso-guanidine (NTG) and low energy ion beam implantation and optimizing the fermentation medium. Mutation resulted in 1.66fold higher production of vitamin K2 than that of the parentl strain. The production by the mutant BN-P15-11-1 was increased 55% and reached 3.593±0.107 mg/L by using the Placke...

L-Glutaminase is widely distributed in microorganisms including bacteria, yeast and fungi. The enzyme mainly catalyzes the hydrolysis of γ-amido bond of l-glutamine. In this report medium optimization was conducted through one -factor -at -a -time approach for the submerged production of L-Glutaminase by Streptomyces griseus using different additional carbon, nitrogen, amino acids, mineral salt...

METHODS A 2.8M phosphate buffer is prepared by dissolving 21.7 Gm. dibasic potassium phosphate (K2HPO4) and 16.9 Gm. monobasic potassium phosphate (KH2PO4) in distilled water free of CO2. The volume is adjusted to 100 ml. To 1.8 ml. of this buffer, 20 mg. of sodium hydrosulfite (Na2S2O4) and 0.2 ml. of buffered hemolysate (3) are added in a small test tube. After 15 minutes or longer the mixtur...

An actinomycete strain was isolated from northern part of Bangladesh and identified as a new Streptomyces species on the basis of its morphological, biochemical, cultural characteristics and 16S rRNA data. Attempts were made to optimize the culture conditions for the production of antimicrobial metabolites by this strain. Antimicrobial metabolites production was started after 7 days of incubati...

Alpha-galactosidase production in submerged fermentation by Acinetobacter sp. was optimized using feed forward neural networks and genetic algorithm (FFNN-GA). Six different parameters, pH, temperature, agitation speed, carbon source (raffinose), nitrogen source (tryptone), and K2HPO4, were chosen and used to construct 6-10-1 topology of feed forward neural network to study interactions between...

We designed phosphonium-type zwitterion (ZI) to control the saturated water content of separated ionic liquid (IL) phase in the hydrophobic IL/water biphasic systems. The saturated water content of separated IL phase, 1-butyl-3-methyimidazolium bis(trifluoromethanesulfonyl)imide, was considerably improved from 0.4 wt% to 62.8 wt% by adding N,N,N-tripentyl-4-sulfonyl-1-butanephosphonium-type ZI ...

Municipal waste is one of the most hazardous components of developing countries. However, enzymes do provide an eco-friendly solution in this case. Amylase is an important enzyme in food, textile and pharmaceutical industry and can be used for bioconversion of waste. From the municipal solid waste we have isolated an amylase producing bacteria that can grow in the irritant municipal waste and h...