Deubiquitinating enzymes (DUBs) are a largely understudied and untapped resource in the toolkit of protein degradation functionalities. They comprise large repertoire that remove ubiquitin from substrates variety cellular pathophysiological contexts, have enormous potential for research clinical use. It is only within last 5 years potent, selective, well-characterized small-molecule inhibitors ...

Degrader technologies, which enable the chemical knockdown of disease-causing proteins, are promising for drug discovery. After two decades research, degraders using ubiquitin-proteasome system (UPS) currently in clinical trials. However, UPS substrates mainly limited to soluble proteins. Autophagy-targeting chimeras and autophagosome-tethering compounds that use autophagy, has functions comple...

Induced protein degradation accomplishes elimination, rather than inhibition, of pathological proteins. Key to the success this novel therapeutic modality is modification proteins with ubiquitin chains, which brought about by molecular glues or bivalent compounds that induce proximity between target and an E3 ligase. The human genome encodes ?600 ligases differ widely in their structures, catal...

We present the implementation of Distributed Constructionism (DC) through a Participatory Design (PD) methodology for an Online Learning community. The students collaborate on the content and functionality development of an online Modern Greek language course, peer review and publish content contributions, and participate in participatory design teams. Participatory design was implemented as a ...