We used an ITS2 primary and secondary structure and Compensatory Base Changes (CBCs) analyses on new French and Spanish Dunallela salina strains to investigate their phylogenetic position and taxonomic status within the genus Dunaliella. Our analyses show a great diversity within D. salina (with only some clades not statistically supported) and reveal considerable genetic diversity and structur...

Internal transcribed spacer (ITS) regions of the eukaryotic rDNA operon are integral to the correct processing and maturation of rRNAs. To further understand the evolution of this region, we elucidated the secondary structure of ITS2 from representatives of the eight divergent clades of Symbiodinium Freud., a large genus of dinoflagellate endosymbionts occurring in association with zooxanthella...

Food-borne fluke infections/trematodiases are emerging as a major public health problem worldwide with over 40 million people affected and over 10% of world population at risk of infection. The major concentration of these infections is in Southeast Asian and Western Pacific Regions, where the epidemiological factors (including the prevalent socio-cultural food habits) are conducive for transmi...

samples were collected from soil and as well from phyllosphere of rice plant and subjected to dilution plate technique on rbsf trichoderma selective medium. a total of 202 trichoderma isolates were obtained from rice fields at different geographical locations distributed all over mazandaran province. single spore isolates were obtained and genomic dna extracted from all the isolates using dna e...

ticks are important acarina that infest animals. they are obligatory blood sucker arthropods which economically impact cattle industry by reducing weight gain and production. moreover, they are important vectors of viral, bacterial, rickettsial and parasitic pathogens infecting humans and animals. in view of the importance of hyalomma anatolicum anatolicum in pathogen transmission, including th...

Polygalaceae is a large family with cosmopolitan distribution, comprising ca. 1,200 species in 27 genera. However, similar to many plant groups, the identification of mostly depends on floral and fruit characteristics; therefore, DNA barcoding could easily warrant corect sterile material. In current study, utility six widely employed barcode loci, namely rbcL, matK, trnL-F region (including trn...

Green algae, Chlorella ellipsoidea, Haematococcus pluvialis and Aegagropila linnaei (Phylum Chlorophyta) were simultaneously decoded by a genomic skimming approach within 18-5.8-28S rRNA region. Whole genomic DNAs were isolated from green algae and directly subjected to low coverage genome skimming sequencing. After de novo assembly and mapping, the size of complete 18-5.8-28S rRNA repeated uni...

An increasing number of phylogenetic analyses are based on the internal transcribed spacer 2 (ITS2). They mainly use the fast evolving sequence for low-level analyses. When considering the highly conserved structure, the same marker could also be used for higher level phylogenies. Furthermore, structural features of the ITS2 allow distinguishing different species from each other. Despite its im...