Curcuma wenyujin is an important multifunctional medicinal herb in China. Currently, populations of C. wenyujin are decreasing, and wild individuals have almost disappeared from their natural habitats. Moreover, little is known regarding the molecular characteristics of this plant. In this study, we investigated the genetic diversity and variation of five populations of C. wenyujin, using ran-d...

Genetic diversity and differentiation of 50 Colletotrichum spp. isolates from legume crops studied through multigene loci, RAPD and ISSR analysis. DNA sequence comparisons by six genes (ITS, ACT, Tub2, CHS-1, GAPDH, and HIS3) verified species identity of C. truncatum, C. dematium and C. gloeosporiodes and identity C. capsici as a synonym of C. truncatum. Based on the matrix distance analysis of...

The recently developed Inter-Simple Sequence Repeat PCR (ISSR-PCR) or microsatellite primed PCR or Simple Sequence Repeat (SSR)-Anchored PCR technique detects polymorphic markers in a wide variety of genomes. Usually the ISSR primers are either 5' end-labeled with gamma[32P]ATP or one of the alpha[32P] labeled dNTPs is added to the PCR reaction and the PCR products are resolved on PAGE and auto...

Previous studies based on DNA restriction-site and sequence variation have shown that the Hawaiian lobeliads are monophyletic and that the two largest genera, Cyanea and Clermontia, diverged from each other ca. 9.7 Mya. Sequence divergence among species of Clermontia is quite limited, however, and extensive hybridization is suspected, which has interfered with production of a well-resolved mole...

Molecular markers derived from polymerase chain reaction (PCR) amplification of genomic DNA are an important part of the toolkit of evolutionary geneticists. Random amplified polymorphic DNA markers (RAPDs), amplified fragment length polymorphisms (AFLPs) and intersimple sequence repeat (ISSR) polymorphisms allow analysis of species for which previous DNA sequence information is lacking, but do...

Drought is one of the most important abiotic stress factors affecting crop yields and qualities worldwide. One drought-sensitive plant cucumber, which has a high transpiration rate. Grafting recognized as promising approach to increasing tolerance stresses in cucumber. In this study, it aimed determine rootstock that will prevent negative changes some growth, physiological genetic parameters cu...

In this study Artemisia amygdalina a critically endangered medicinal plant endemic to Kashmir Himalayas was grown under in vitro conditions. ISSR markers were used to study the somaclonal variation at the genetic level. Two ISSR-primers (CAC)3 GC and (ACTG)5 were used, two additional DNA bands (0.15kb and 0.2kb) were observed respectively in tissue cultured Artemisia amygdalina. Direct regenera...

tumor necrosis factor alpha-inducible protein 3 (tnfaip3) gene polymorphisms have been reported to be associated with the susceptibility to several immune-related diseases. here we investigated the effect of tnfaip3 gene polymorphisms on the risk of allergic rhinitis (ar) in a chinese han population. the case-control study included 540 ar patients and 524 healthy controls. genotyping for tnfaip...

The work involved a molecular biological technique (ISSR-PCR) using ISSR-primer S-2, with structure (AGC) 6G. Changes in the gastric mucosa chronic duodenal ulcer disease against background of severe atrophic gastritis have been analyzed. Noteworthy is fact that there strong correlation between degree dysplasia epithelium and mitotic index, Pearson's coefficient rxy was 0.853, respectively. A v...

Sequence-related amplified polymorphism (SRAP), amplified fragment length polymorphism (AFLP), and inter-simple sequence repeat (ISSR) markers were used to estimate the genetic diversity and relationships among Eucommia ulmoides cultivars in China. A total of 240, 192, and 150 DNA fragments were detected by 10 SRAP primer combinations, 10 AFLP primer combinations, and 10 ISSR primers, among whi...