The properties of the microbody malate dehydrogenase (EC 1.1.1.37) (MDH) isozyme from cotyledons of Cucumus sativus L. were compared during development. It is concluded that the isozyme remains unaltered, despite the transition from glyoxysomal to peroxisomal function that occurs during greening of the cotyledons. This conclusion is based on electrophoretic behavior, chromatographic elution fro...

Normal feline and human arylsulfatase B isozymes were purified to homogeniety from liver. The specific activities of the feline and human enzymes toward p-nitrocatechol sulfate were 1,100,000 and 800,000 units/mg of protein, and toward UDP-N-acetylgalactosamine-4-sulfate were 5,500 and 4,000 units/mg of protein, respectively. Although both enzymes had the same pH optimum (5.7), the feline enzym...

This study evaluates the relative contributions of envelope and fine structure cues in both temporal and spectral domains to Mandarin tone recognition in quiet and in noise. Four sets of stimuli were created. Noise-excited vocoder speech was used to evaluate the temporal envelope. Frequency modulation was then added to evaluate the temporal fine structure. Whispered speech was used to evaluate ...

Two years ago Markert and Mbller (1959) introduced the concept of an isozyme, which they defined as one of the different molecular forms of an enzyme. They proposed that each isozyme could be controlled by a separate gene or that an isozymic series could be controlled by a single gene. Essentially the problem centers on the way in which genes control a protein: how a protein is made and how it ...

Postnatal developmental changes of lactate dehydrogenase (LDH : EC 1.1.1.27), malate dehydrogenase and asparate transferase in the nervous tissue of rats have been reviewed by Bonavita (1), and normal topochemical distribution of LDH isozyme in human adult brain was investigated by Gerhardt et al. (12). In recent years, the LDH isozyme in peri-implantation embryos of various animals was studied...

Restriction endonuclease analysis of mtDNA was used to examine the genetic relatedness of several geographically separated isolines of the Drosophila mercatorum subgroup. In addition, we examined the temporal and spatial distribution of two mtDNA restriction site polymorphisms produced by the enzymes BstEII and BstNI at a single locality--Kamuela, Hawaii. Due to small sample sizes of some colle...

hydatidosis is a disease caused by infection with the metacestode stage of the dog tapeworm,echinococcus granulosus. this is recognised as one of the world’s most important zoonoses, affecting bothhumans and their domestic animals. in the current study, 20 sheep cystic livers or lungs were collected.hydatid fluid and protoscolices were isolated and the soluble protein was prepared. polypeptide ...

The first step in metabolism of glucose (Glc) is usually phosphorylation, catalyzed by hexokinase. However, the Glc-6-P produced can then enter one or more of several alternative pathways. Selective expression of isozymic forms of hexokinase, differing in catalytic and regulatory properties as well as subcellular localization, is likely to be an important factor in determining the pattern of Gl...