نتایج جستجو برای: isoelectric focusing

تعداد نتایج: 103945  

Journal: :Applied and environmental microbiology 1997
K Venema M L Chikindas J Seegers A J Haandrikman K J Leenhouts G Venema J Kok

The bacteriocins lactococcin B and pediocin PA-1 were purified by ethanol precipitation, preparative isoelectric focusing, and ultrafiltration. The procedure reproducibly leads to high final yields in comparison to the generally low yields obtained by column chromatography. Specifically, during isoelectric focusing no loss of activity occurs. The method, in general, should be applicable to smal...

2007
C. WANG

Isoelectric points of Kunitz' crystalline soybean trypsin inhibitor (STl), Bowman-Birk STI, and STI prepared by chromatography on diethylaminoethyI (DEAE)-cellulose were investigated by isoelectric focusing, gel filtration, ion-exchange resin treatment, solubility test, and titration. Results suggest that the STI of Kunitz may have an isoelectric point below pH 4.5. The isoelectric focusing pat...

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