Dendritic cells (DCs) use cellular pathways collectively referred to as cross-presentation to stimulate CD8 + T cells with peptide Ags derived from internalized, exogenous Ags. We have recently reported that DCs rely on aminoterminal trimming of cross-presented peptides by insulin-responsive aminopeptidase (IRAP), an enzyme localized in a regulated endosomal storage compartment. Considering a r...

Inter leukinla and interleukin-ip are two polypeptides which share a significant number of inflammatory, immunological and pathological properties (for a review see [1]). Importantly, these dissimilar 17 kDa proteins bind to two classes of interleukin-1 receptors, resulting in the mediation of several immune and inflammatory responses and in the induction of a variety of biological changes in n...

The environment can have a decisive influence on the structure of the genome, changing it in a certain direction. Therefore, the genomic distribution of environmentally sensitive transposable elements may vary measurably across a species area. In the present research, we aimed to detect and evaluate the level of LTR retrotransposon intraspecific variability in Aegilops speltoides (2n = 2x = 14)...

The multiclass Lighthill-Whitham-Richards (MCLWR) traffic model, which distinguishes N classes of drivers differing in preferential velocity, gives rise to a system of N strongly coupled, nonlinear first-order conservation laws for the local car densities as a function of distance and time.We propose a new class of anti-diffusive schemes by splitting the system of conservation laws into two dif...

Insulin-responsive trafficking of the GLUT4 glucose transporter and the insulin-regulated aminopeptidase (IRAP) in adipose and muscle cells is well established. Insulin regulation of GLUT4 trafficking in these cells underlies the role that adipose tissue and muscle play in the maintenance of whole body glucose homeostasis. GLUT4 is expressed in a very limited number of tissues, most highly in a...

Background: Hyperglycemia or hyperinsulinemia contributes to poorer endometrial cancer survival. It was shown that P-LAP/IRAP translocates to the plasma membrane in response to insulin stimulation. Recently, we demonstrated that P-LAP/IRAP is associated with a poor prognosis in endometrial adenocarcinoma patients. The aim of this study was to examine whether the malignant potential of endometri...

Presentation of exogenous antigens on MHC class I molecules, termed cross-presentation, is essential for the induction of CD8 T-cell responses and is carried out by specialized dendritic cell (DC) subsets. The mechanisms involved remain unclear. It has been proposed that antigens could be transported by endocytic receptors, such as the mannose receptor (MR) in the case of soluble ovalbumin, int...

The stability of aging barley calli was investigated with the barley retroelement 1 (BARE-1) retrotransposon specific inter-retrotransposon amplified polymorphism (IRAP) technique. Mature embryos of barley (Hordeum vulgare cv. Zafer-160) were cultured on callus induction MS medium supplemented with 3 mg/L 2,4-D and maintained on the same medium for 60 days. Ten IRAP primers were used in 25 diff...

The Implicit Relational Assessment Procedure (IRAP) examines implicit attitudes through the measurement of response latencies. In this study, the IRAP was used to assess implicit attitudes toward “mainstream” sexual terms (e.g., Kissing) and “BDSM” terms (e.g., Bondage) among individuals reporting BDSM interests and among students who did not report such interests. A questionnaire was used to m...