In this communication we discuss the development of ionophore based nanosensors for the detection and monitoring of histamine levels in vivo. This approach is based on the use of an amine-reactive, broad spectrum ionophore which is capable of recognizing and binding to histamine. We pair this ionophore with our already established nanosensor platform, and demonstrate in vitro and in vivo monito...

The effects of vasopressin, angiotensin II, and calcium ionophore A23187 were examined and compared on endogenous prostaglandin E2 (PGE2) production, in the absence of exogenous arachidonate, by glomeruli and cortical and medullary collecting tubules freshly prepared from rat kidney. Angiotensin II stimulated PGE2 production in the glomeruli but not in the collecting tubules. Vasopressin at con...

A fluorescence technique that measures fast ion fluxes across liposome membranes was used to determine the molecularity of the colicin E1 channel. The rate of flux of Tl+ (used as a K+ analogue) into large unilamellar vesicles was measured by its ability to quench the fluorescence of a membrane-impermeable fluorophore entrapped in the vesicles. The dependence of Tl+ flux rate on the concentrati...

Metal-carboxylic ionophore complexes, where Metal = Ag, Tl, Li, Na, K, Rb, Mg, Mn, Cs, Sr, Ba, Cu and Ionophore = monensin (MON-H), have been synthesized. The molecular structures of three complexes have been solved by X-ray diffraction methods. The metal chelates were tested against human tuberculosis line for potential anti-tubercular activity against Mycobacterium tuberculosis H37Rv (MTB). T...

Sarcoplasmic reticulum vesicles and purified ATPase were studied by fluorescence spectroscopy. The preparations exhibited similar excitation and emission spectra, characteristic of tryptophan fluorescence. Addition of calcium to the preparations suspended in ethylene glycol bis(P-aminoethyl ether)N, N, N’, N’-tetraacetic acid (EGTA) solutions promoted a 3 to 4% increase in fluorescence intensit...

Salinomycin is a carboxylic polyether ionophore which was isolated from the culture super‐ natant of the bacterium Streptomyces albus in 1974 [1]. Structurally, it is composed of a penta‐ cyclic molecule with a unique tricyclic spiroketal ring system and a unsaturated sixmembered ring (Fig. 1). Its lipophilic property enables salinomycin to act in cytoplasmic and mitochondrial membranes as an i...

In this study, a new potentiometric sensor selective to copper(II) ions was developed and characterized. The has polymeric membrane contains 4.0% electroactive material (ionophore), 33.0% poly(vinyl chloride) (PVC), 63.0% bis(2–ethylhexyl)sebacate (BEHS) 1.0% potassium tetrakis(<italic>p</italic>–chlorophenyl)borate (KT<italic>p</italic>ClPB). This novel copper( II)–sele...

Two taste preference studies were conducted using six Holstein heifers in each experiment to determine preferences for no ionophore, lasalocid, or monensin in the diet. In Exp. 1, individually penned (approx. 5 mo old; 220 +/- 14 kg BW) heifers were fed a basal total mixed ration containing 46% corn silage, 46% grass haylage, and 8% soybean meal (DM basis). There were five treatments (mg/kg BW(...

Four sesquiterpenes isolated from Jasonia glutinosa D.C. (Asteraceae), namely lucinone, glutinone, 5-epi-kutdtriol and kutdtriol, have been evaluated for their in vitro anti-inflammatory activity in cellular systems generating cyclooxygenase (COX) and 5-lipoxygenase (5-LOX) metabolites. None of the compounds assayed had a significant effect on leukotriene C4 (LTC4)-release from calcium ionophor...

The luteinizing hormone-releasing hormone (LHRH) agonist ICI 118630 was found to increase testosterone production in purified rat testis Leydig cells in a concentration- and time-dependent manner, but no consistent changes in cyclic AMP levels were detectable. The stimulation of steroidogenesis by LHRH agonist was found to be dependent on the concentration of Ca2+ in the incubation medium; at l...