Routine manipulation of cellular genomes is contingent upon the development of proteins and enzymes with programmable DNA sequence specificity. Here we describe the structure-guided reprogramming of the DNA sequence specificity of the invertase Gin from bacteriophage Mu and Tn3 resolvase from Escherichia coli. Structure-guided and comparative sequence analyses were used to predict a network of ...

Contrasting evidence has accumulated regarding the role of acid invertase and sucrose synthase in tomato fruit sink establishment and maintenance. In this work the relationships among the activities of sucrose synthase and acid invertase, Lycopersicon esculentum Mill cv UC-82B fruit growth, and starch accumulation were analyzed in fruit at 0 to 39 d after anthesis. Sucrose synthase, but not aci...

Fruits of orange-fleshed and green-fleshed muskmelon (Cucumis melo L.) were harvested at different times throughout development to evaluate changes in metabolism which lead to sucrose accumulation, and to determine the basis of differences in fruit sucrose accumulation among genotypes. Concentrations of sucrose, raffinose saccharides, hexoses and starch, as well as activities of the sucrose met...

In the present work, a continuous microreactor was designed to study the effect of miniaturization on bioreactors for the production of invertase from S. cerevisiae MM01 in a microfluidic environment. In order to simplify the analysis and considering typical production strategies at laboratory scale, 250 ml baffled flasks and a 2.5 l batch bioreactor were used as bench-scale models for the perf...

The effect of NaCl and ABA on germination, sugars and enzymes of carbohydrate metabolism in sorghum embryos and endosperm was investigated. Both phytohormone and salt stress influenced different aspects at physiological and biochemical level. Germination decreased markedly under NaCl and ABA treatments. Subsequently, an influence of salt stress and ABA on total and reducing sugars along with am...

Most recent publications describing the properties of invertase refer to enzyme preparations obtained from plants, bacteria, fungi, protozoa or the honey bee (Edelman, 1956; Allen & Bacon, 1956; Pavlinova & Kursanov, 1956; Christie & Porteous, 1957; Carnie & Porteous, 1959a; Howard, 1959). Borgstrom & Dahlqvist (1958), Dahlqvist & Borgstrom (1959) and Dahlqvist (1958, 1959a-c, 1960a-f, 1961) ha...

Sugar-end defect is a tuber quality disorder and persistent problem for the French fry processing industry that causes unacceptable darkening of one end of French fries. This defect appears when environmental stress during tuber growth increases post-harvest vacuolar acid invertase activity at one end of the tuber. Reducing sugars produced by invertase form dark-colored Maillard reaction produc...

Seed coating is a technology to improve germination and homogenize stand establishment. Although coating often results in lower germination rates, seeds that do germinate grow more vigorously and show strongly reduced respiratory losses during reserve mobilization. We hypothesize that the higher mobilization efficiency is due to a shift in the enzymatic cleavage of sucrose from invertase to suc...

Agro-industrial wastes have been used as substrate-support in solid state fermentation for enzyme production. Molasses and sugarcane bagasse are by-products of sugar industry and can be employed as substrates for invertase production. Invertase is an important enzyme for sweeteners development. In this study, a xerophilic fungus Aspergillus niger GH1 isolated of the Mexican semi-desert, previou...

The main goal of this work was the production and characterization of a novel invertase activity from Zygosaccharomyces bailii strain Talf1 for further application to biodesulfurization (BDS) in order to expand the exploitable alternative carbon sources to renewable sucrose-rich feedstock. The maximum invertase activity (163 U ml(-1)) was achieved after 7 days of Z. bailii strain Talf1 cultivat...