A method for the extraction and quantitation of 3H inulin from tissues is described. This method does not require either combustion technics or time-consuming acid extraction procedures. Inulin is as soluble in the liquid scintillation medium as it is in water. Therefore, hot-water tissue extracts without further treatment were employed to quantitate l1 inulin. Nearly 100% recovery was obtained...

Simultaneous determination of glomerular filtration rate by inulin clearance may be required during studies of the renal excretion of the bacterial polysaccharide, dextran. This involves the estimation of both inulin and dextran in the same plasma and urine samples. Unfortunately, dextran interferes with the analysis of inulin, both by the method of Alving, Flox, Pitesky, and Miller (1) and by ...

introduction: a mixture of wheat flour, water, salt, yeast and other optional ingredients such as preservatives, enzymes, whey powder, surfactants, sugar, fibres and improvers form bread dough. enrichment of dough and bakery products by dietary fibres such as wheat bran and resistant starch, beta-glucans and inulin has been reported (peressini, & sensidoni, 2009; hager, ryan, schwab, gänzle, o’...

Complex fermentable carbohydrates, such as inulin-type fructans have been shown to improve Mg absorption in the hindgut and body stores. The mechanisms for this are not well understood. The newly identified transient receptor potential melastatin 6 and 7 (TRPM6 and TRPM7) channels have been shown to function in active epithelial Mg transport in the apical membrane of epithelial cells, the kidne...

We evaluated urinary excretion and tubular transport of H-digoxin by three different methods in anesthetized rats made diuretic by infusion of 2.5% saline. In one group small volumes of H-digoxin and "C-inulin were injected simultaneously into surface proximal convolutions, and urine was collected serially from both ureters. Digoxin recovery was lower after early (62.1 ± 5.3%) than after late (...

Postischemic acute renal failure (ARF) induced by cardiac surgery is commonly prolonged and may be irreversible. To examine whether persistence of postischemic, tubular cell injury accounts for delayed recovery from ARF, we studied 10 patients developing protracted (36 +/- 4 d) ARF after cardiac surgery. The differential clearance and excretion dynamics of probe solutes of graded size were dete...

BACKGROUND previous studies have shown that non-digestible inulin-type fructan intake can increase intestinal mineral absorption in both humans and animals. However, this stimulatory effect on intestinal absorption may depend on experimental conditions such as duration of fermentable fiber intake, mineral diet levels and animals' physiological status, in particular their age. OBJECTIVES the a...

It has been confirmed that Saccharomyces sp. W0 can produce high concentration of ethanol. In this study, the INU1 gene cloned from the marine-derived Pichia guilliermondii was transformed into uracil mutant of Saccharomyces sp. W0. The positive transformant Inu-66 obtained could produce 34.2 U ml⁻¹ of extracellular inulinase within 72 h of cultivation. It was found that 15.2 U of inulinase act...

This study is oriented on the preparation of lactic acid fermented cabbage juices with addition of inulin preparation FRUTAFIT®. Vegetable juices were inoculated by lactic acid bacteria: Lactobacillus amylovorus, CCM 4380, Lactobacillus amylophilus CCM 7001, CCM 7039 Lactobacillus plantarum, Bifidobacterium longum CCM 4990, and a mixture of Lactobacillus plantarum CCM 7039 and Bifidobacterium l...

OBJECTIVE Since the conventional drip-infusion method for measuring inulin clearance (Cin) has problems related to its accuracy and performance, we explored a more accurate and concise method by rapid intravenous injection of a newly developed inulin fraction (Inulead(®)), in which spot urine sampling was omitted and the administration period of inulin was shortened from 120 to 5 minutes. PAT...