An interferon-stimulated response element (ISRE)/interferon regulatory element (IRE) spanning nucleotide coordinates 1091-1100 is present in the enhancer 1/X gene promoter region of the hepatitis B virus (HBV) genome. In the context of a minimal promoter element, the enhancer 1/X gene promoter ISRE/IRE was shown to be a functional regulatory site capable of mediating interferon alpha- (IFNalpha...

A self-regulated high-copy number plasmid containing chloramphenicol resistant gene, for the production of recombinant proteins under the regulation of bacteriophage ?pL promoter, was constructed. The designed 5024 base pair expression plasmid contained a heat sensitive repressor cI857 coding gene to regulate the function of ?pL promoter under heat shock induction. Using the constructed vector,...

BACKGROUND Toll-like receptors (TLRs) and macrophages play an important role in rheumatoid arthritis (RA). Currently, it is not clear whether inflammatory M1 or anti-inflammatory M2 predominate among the resident macrophages in the synovium. In the present study, we set out to investigate the impact of TLR stimulation on monocyte-derived M1 and M2 macrophage function and phenotype by mimicking ...

Mouse myeboidleukemic Ml cells were induced to differen tiate in vitro into macrophages and granubocytes by various inducers, such as differentiation stimulating factor (D-factor) in conditioned medium of mouse peritoneal macrophages and mouse embryo cells, dexamethasone, bipopolysaccharide, and polyinosinic acid. Interferon, which was prepared from Ml cells treated with copolymer of pobyinosin...

The persistence of immunity to malaria induced in mice by a heterologous DNA priming and poxvirus boosting regimen was characterized. Mice were immunized by priming with DNA vaccine plasmids encoding the Plasmodium yoelii circumsporozoite protein (PyCSP) and murine granulocyte-macrophage colony-stimulating factor and boosting with recombinant vaccinia encoding PyCSP. BALB/c mice immunized with ...

Patients with diffuse large cell lymphoma who have an adequate vitamin D supply derive significantly more benefit from immuno-chemotherapy rituximab than patients deficiency; this is especially true for female patients. We already been able to show that increases the antibody-dependent cytotoxicity (ADCC) of NK cells in a sex-dependent manner, but it unclear how makes efficient. Methods: Health...

background: interferons (ifns) are common therapeutics for several diseases such as viral hepatitis, multiple sclerosis and malignancy. a variety of autoimmune related side effects have been observed during ifn therapy. rare cases of myopathy, polymyositis or dermatomyositis have been reported during therapy with high doses of ifn^5. case presentation: in this report, we describe a case with se...

We have introduced the human beta-interferon gene with its promoter region into murine B-cell and fibroblast cell lines via a Moloney murine leukemia virus (M-MuLV) vector and have studied the inducible expression of the beta-interferon gene as a function of the various retroviral vector designs. By deleting the enhancer within the 3' viral long terminal repeat (LTR), inserting the human beta-i...

Sézary syndrome is a cutaneous T-cell lymphoma characterized by the triad of erythroderma, generalized lymphadenopathy, and the presence of Sézary cells in skin, lymph nodes and peripheral blood. A 59-year-old Chinese female presented with erythroderma, palmoplantar keratoderma and enlarged right axillary and epitrochlear lymph nodes. Staging investigations showed extensive involvement of perip...