We present a novel method to form more than 10,000 asymmetric lipid-bilayer membranes at a time on micro-chamber arrays. In our method, the arrayed asymmetric lipid-bilayers, where lipid compositions are different between inner and outer leaflets, are formed by injecting several kinds of liquids into a micro-device that has hydrophilic-in-hydrophobic surfaces. The lipid compositional asymmetry ...

The heavy chain of myosin can be detected in human red cell membranes by immunoblot analysis with antiplatelet myosin antibodies. Neonatal red cell membranes have more immunoreactive myosin than adult membranes. Membranes from young adult red cells contain more immunoreactive myosin than membranes from old adult red cells. In contrast, young and old neonatal red cells have equivalent mounts of ...

Signal recognition particle (SRP), an 11S ribonucleoprotein (Walter and Blobel (1982) Nature 299, 691-698), is required for translocation of secretory proteins across microsomal membranes (Walter and Blobel (1980) Proc. Natl. Acad. Sci. USA 77, 7112-7116) and for asymmetric integration into microsomal membranes of a transmembrane protein (Anderson et al., (1982) J. Cell Biol. 93, 501-506). We d...

It is well-established that native plasma membranes are characterized by an asymmetric distribution of charged (anionic) lipids across the membrane. To clarify how the asymmetry can affect membrane electrostatics, we have performed extensive atomic-scale molecular dynamics simulations of asymmetric lipid membranes composed of zwitterionic (phosphatidylcholine (PC) or phosphatidylethanolamine (P...

Asymmetrically shaped nanopores have been shown to rectify the ionic current flowing through pores in a fashion similar to a p-n junction in a solid-state diode. Such asymmetric nanopores include conical pores in polymeric membranes and pyramidal pores in mica membranes. We review here both theoretical and experimental aspects of this ion current rectification phenomenon. A simple intuitive mod...

( Asymmetric cellulose acetate membranes have been prepared and characterized in the laboratory with a view to use them in membrane bio-reactors. Enzymatic hydrolysis of sucrose has been investigated in a dead-end stirred cell type membrane bio-reactor using these membranes. Effect of different operating variables such as substrate concentration, enzyme loading, flow rate and working volume hav...