OBJECTIVE To investigate and compare the protective impact of the in situ formed salivary pellicle on enamel and dentine erosion caused by different acids at pH 2.6. METHODS Bovine enamel and dentine samples were exposed for 120 min in the oral cavity of 10 healthy volunteers. Subsequently, enamel and dentine pellicle-covered specimens were extraorally immersed in 1 ml hydrochloric, citric or...

Aim of this study was to investigate the distribution of versican proteoglycan within the human dentine organic matrix by means of a correlative immunohistochemical analysis with field emission in-lens scanning electron microscope (FEI-SEM), transmission electron microscope (TEM), fluorescence microscope (FM) and biochemical assay. Specimens containing dentine and predentine were obtained from ...

A dentine protein extraction protocol was modified in order to identify matrix metalloproteinase gelatinolytic activities in the non-mineralized and mineralized phases of human dentine. Dentine proteins from 24 individual permanent molars from patients aged 15-73 years were sequentially extracted, first with guanidinium chloride (G1 extract), then EDTA (E extract), and after this demineralizati...

OBJECTIVE The aim of the present study is to investigate the ultrastructural changes within the different zones of carious dentine and compare those changes with sound dentine. METHODS Transmission electron microscopy and electron diffraction techniques were used to investigate the effect of caries on the inorganic phase of dentine. Areas of interest were identified with optical and scanning ...

This in vitro study evaluated the effect of commercially available remineralising products on demineralised dentine. Demineralised dentine was stored in pooled saliva or treated with Tooth Mousse, Tooth Mousse Plus, Gel-Kam or NeutraFluor 5000, for 7 days at room temperature. Dentine slices were microhardness tested, and assessed using scanning electron microscopy (SEM) and energy dispersive an...

OBJECTIVE A morphological and ultra-structural study of copper vapor laser (lambda = 510.6 nm) effects on enamel and dentine was performed to show the effects of this radiation. METHODS A total of 15 human molars were cut in half; 15 pieces were separated for irradiation on enamel and 15 for dentine. These two groups were further divided into five experimental groups, including a control grou...

This study was performed to examine the possibility of Er:YAG laser for dentine hypersensitivity treatment using a novel laser probe; broom-type probe. The morphological change of dentinal tubules of bovine dentine plate after low power laser irradiation (5 or 10 mJ/ pulse, 10 pps) or boiling was observed by SEM. Fifty teeth from 13 patients aged 31–54 years with complaint of dentine hypersensi...

Data have been presented indicating that changes occur in the dental structures of growing albino rats fed diets adequate except for extreme restriction of inorganic salts (Arnim et al.2). Marked rarefaction of the alveolar bone and the formation of irregular dentine containing few tubules, many spaces, and occasional cells were observed. This unusual dentine was most prominent in the cusps of ...

Root caries prevention has been a challenge for clinicians due to its special anatomical location, which favors the accumulation of dental plaque. Researchers are looking for anti-biofouling material to inhibit bacterial growth on exposed root surfaces. This study aimed to develop polydopamine-induced-polyethylene glycol (PEG) and to study its anti-biofouling effect against a multi-species cari...

A method for the preparation of circumpulpal dentine for lead analysis is described. The technique consists of cutting 6oo-,L-thin sections of deciduous teeth, and, utilizing a vice, fracturing off the circumpulpal dentine from the coronal dentine. Both dentine and circumpulpal dentine of teeth of normal American children and previously identified lead-poisoned children were prepared in this fa...