Polygalacturonase is a valuable biocatalyst for several industrial applications. Production of polygalacturonase using the Aureobasidium pullulans stain isolated from Saharan soil of Algeria was investigated. Its capacity to produce polygalacturonase was assessed under submerged culture using tomato pomace as an abundant agro-industrial substrate. Optimization of the medium components, which en...

besides the high starch content, sweet potato contains endogenous amylases where the two predominants are - and β-amylases. sweet potato is thought to be a promising source of β -amylase since β -amylase is one of the major protein in the tubers. this investigation has focused on the properties of β amylase from white-flesh sweet potato that has been grown in iran as a potential source for indu...

In recent years, several oconjugation protocols have been developed to improve the functional properties of the enzyme lysozyme. In this study, the optimum conditions of lysozyme – oxidized inulin conjugation and functional properties of the modified lysozyme were investigated. Initially, inulin (MW about 25 kDa) was oxidized by periodate to provide the reactive carbonyl groups  to attach to am...

Background: Phenolic compounds, which are produced routinely by industrial and urban activities, possess dangers to live organisms and environment. Laccases are oxidoreductase enzymes with the ability of remediating a wide variety of phenolic compounds to more benign molecules. The purpose of the present research is surface display of a laccase enzyme with adhesin involved in diffuse adhesion (...

Alpha-amylase is widely used as an industrial enzyme, and a therapeutic target for which inhibitors are designed. Organic solvents are used to dissolve various compounds that would be studied as moderators of alpha-amylases, but they could themselves affect enzyme activity and stability. Methanol, ethanol and propanol are simple alcohols that may be commonly used to this end, and their effect h...

The increase of environmental pollutants especially heavy metals derives from human industrial communities. Zinc is one of the natural elements that exist in biotic environment, plants body, and animals in different forms. Zinc has toxic and lethal effects in high concentrations on plants. Also some plants are introduced as accumulators of this element. Therefore, an investigation was carried o...

A thermophilic strain AMF-07, hydrolyzing carboxymethylcellulose (CMC) was isolated from Kerman hot spring and was identified as Bacillus licheniformis based on 16S rRNA sequence homology. The carboxymethylcellulase (CMCase) enzyme produced by the B. licheniformis was purified by (NH4)2SO4 precipitation, ion exchange and gel filtration chromatography. The purified enzyme gave a single band on S...

Nanotechnology has transformed the science behind many biotechnological sectors, and applied bio-catalysis is not exception. In 2017, enzyme industry was valued at more than 7 billion USD projected to 10.5 by 2024. The laccase an oxidoreductase capable of oxidizing phenolic non-phenolic compounds that have been considered essential tool in fields currently known as white biotechnology green che...

Hairy root systems are formed by transforming plant tissues with the “natural genetic engineer” Agrobacterium rhizogenes. In most plants such as Cichorium intybus L., hairy root cultures have proven to be an efficient system for secondary metabolites production. The effect of Zinc (ZnSO4), a heavy metal, was investigated at different concentrations (0, 1, 5 and 10 mM) on some secondary metaboli...