This study reports on the standardization of an enzyme-linked immunosorbent assay (ELISA) for detecting specific antibodies anti-Trypanosoma cruzi in naturally infected dogs. Sera from 182 mongrel dogs of all ages residing in four rural villages in Santiago del Estero, Argentina, were collected in November 1994 and preserved in buffered neutral glycerin. All sera were tested by indirect hemaggl...

The objectives of this work were to determine the prevalence of Babesia bovis, Babesia bigemina, and Anaplasma marginale detecting antibodies in cattle raised in the semi-arid region of the state of Bahia, Brazil, through indirect enzyme linked immunosorbent assays (ELISA) and to compare the performances of indirect enzyme-linked immunosorbent assays with crude (I-ELISA-CrAnaAg) and recombinant...

Regarding harmful agents in livestock, parasites play an important role moreover it also effect 1/4th of the human populace by various zoonotic diseases (e.g. Toxplasma, Cryptosporidium, Trypanosomiasis, etc.). In livestock the parasites successful invade host immune responses, so early diagnosis of parasitic agents by various immunodiagnostic techniques is very important. Numerous immunologica...

Objectives: Progressive multifocal leukoencephalopathy (PML) is caused by a reactivated infection with the human polyomavirus JC (JCV). Primary infection with JCV has not been linked to any disease in humans. Using recombinant viral protein 1 (VP1) of JCV as antigen, we developed an enzyme linked immunosorbent assay (ELISA) for the detection of VP1-speciŽc IgM. Methods: Two different direct ELI...

We compared washed nasal epithelial cells with unfractionated nasal secretions as sources of respiratory syncytial virus (RSV) antigens in an indirect enzyme-linked immunosorbent assay (ELISA). Of 28 infants positive for RSV by virus isolation or direct immunofluorescence or both, 27 (96%) were positive by ELISA with whole nasal secretions, whereas only 19 (68%) were positive by ELISA with the ...

High levels of seropositivity, in all probability attributable to Ehrlichia, were recorded in the serum of domestic ruminants throughout districts in South Africa where Amblyomma hebraeum, the vector of the heartwater agent, does not occur. The antibodies, detected with the indirect fluorescent antibody (IFA) and the indirect ELISA tests, cross-reacted with Cowdria ruminantium, which was used a...

An enzyme-linked immunosorbent assay (ELISA) for Lawsonia intracellularis was developed and compared with a whole-cell antigen-based immunofluorescence antibody test (IFAT). The antigen-containing lipopolysaccharide (LPS) was derived from Percoll gradient purified cultures of L. intracellularis by using a modification of the Westphal hot phenol procedure. The antigen was bound directly to polys...

EVALUATION OF AN INDIRECT ELISA FOR DETECTION AND TYPING OF FOOT-AND-MOUTH DISEASE VIRUS. An Indirect enzyme linked immunosorbent assay (ELISA) kit was used for diagnosis of foot-and-mouth disease virus (FMDV) types O1, A24, C3 which occurred in Rio Grande do Sul State, Southern Brazil during 1984-1994. The samples were randomly selected and tested by ELISA, Complement Fixation Test (CFT) and i...