A presentation of techniques for organ culture and an evaluation of the effects of organ culture on human and animal corneas is presented. The gross and microscopic appearance of cultured corneas, phase contrast microscopic study, and histopathology of organ-cultured corneas are discussed. These obseivations show that corneas can be stored in organ culture and that after prolonged in vitro pres...

Cell culture technologies form the basis of most alternative methods. They have matured over the last decades, but severe limitations remain, which – to say it positively – leave room for improvement. This second article of the Food for Thought series wants to raise awareness of current limitations that impact on the predictive value of test systems based on cell cultures. The critical question...

background: based on recent studies, there are controversial reports on the ca-pacity of tissue cyst forming of toxoplasma gondii rh strain. in this study, the capac-ity was evaluated by in vivo and in vitro experiments. methods : rh strain was subcutaneously inoculated to ten wistar rats. after one month, their blood, brain, tongue and diaphragm were collected and evaluated by mat, pcr, pathol...

this study was conducted to determine the chemical composition, digestibility, degradability and nutritive value of madder, rubia tinctorum l.  and alfalfa medicago sativa. using in vitro and in situ measurements. the mean values for om, cp, ndf, adf and ee were 892.5, 172.0, 306.5, 219.4 and 16.0 g/kg dm for madder hay, and 921.3, 141.4, 482.0, 361.1 and 6.50 g/kg dm for alfalfa hay, respectiv...

background: based on literature, in vitro cholesterol removal of lactic acid bacteria has been accounted for their in vivo cholesterol reduction. but recently, it has been proposed that such in vitro characteristic may not be directly relevant to their in vivo activity. the objective of this study was to find how much in vitro cholesterol reducing potential of lactobacillus plantarum a7 (la7), ...