Chick embryo extract (CEE) contains a variety of growth factors which may improve in vitro follicle growth. Therefore, the effect of CEE on mouse pre-antral follicle culture was evaluated. Different percentages of CEE (0, 0.50%, 1.00%, 5.00% and 10.00%) were added to culture medium. Hence, the osmolarity of media was measured. Pre-antral follicles with diameter of 120-150 μm were isola...

Lysophosphatidic acid (LPA) known as a serum-derived growth factor, is involved in several cell physiological functions in the female reproductive system including: oocyte maturation, in vitro fertilization and embryo implantation by its transmembrane G protein-coupled receptors. The aim of the present study was to examine the effect of LPA on in vitro follicular development o...

Objective(s): To evaluate the biosynthesis of Ag NPs using plant extract of Melissa officinalis (at the eight leaf stage) grown under in vitro (controlled) condition for the first time.Materials and Methods: Biosynthesis of Ag NPs using plant extract was carried out and formation of Ag NPs confirmed by UV-Visible spectroscopy, X-ray diffraction (XRD), Field Emission Scanning Electron Microscope...

Background: In vitro culture (IVC) of isolated preantral follicles from cryopreserved ovarian tissue might be an efficient method for enhancing mature oocytes in patients who are exposed to infertility. Materials and Methods: Ovaries of 13-day old NMRI mice were removed and randomly placed into control,needle immersed (NIV) and solid surface vitrification (SSV) groups. For vitrification, ovarie...

Nano-fertilizers can increase value of products in agriculture. Iron plays many important and essential roles in plant growth and development as compared to other micronutrients. In the present study, effects of different levels of enriched nano chelated Iron fertilizer (25, 50, 100, and 200 mg l-1)  were investigated in comparison with the common Iron (FeSO4.7H2O) on in vitro proliferation of ...

To develop an alternative method for production of sarsasapogenin by plant tissue culture technique and its quantification in Asparagus racemosus and its in vitro cultures using HPTLC. Murashige and Skoog’s (MS) basal medium supplemented with various growth regulators was used for development of shoot and root calli while, hormone free MS medium was used for development of Agrobacterium tumefac...

Background Background: Mammalian spermatogenesis is regulated through paracrine and endocrine activity, specific cell signaling, and local control mechanisms. These highly specific signaling interactions are effectively absent upon placing testicular cells into two-dimensional primary culture. The specific changes that occur between key cell types and involved spermatogenesis signaling pathways...

Melinacidin is a crystalline mixture of closely related antibacterial agents produced by Acrostalagmus cinnabarinus var. melinacidinus. Melinacidin inhibits a variety of Gram-positive bacteria in vitro but is found to be toxic and ineffective in the treatment of experimental bacterial infections in mice. Melinacidin which inhibits the growth of KB and L-1210 cells in tissue culture appears to b...

Normal mouse mammary epithelial cells from Balb/c mice were successfully cultivated on tissue culture plastic with lethally irradiated LA7 feeder cells. The feeder cells also promoted colony formation from single mouse mammary cells, and the fraction of cells that formed colonies was proportional to the density of feeder cells. The mouse mammary cells could be passaged at least 8-12 times as lo...