A bacterial strain named A216, with feruloyl esterase producing capability, was isolated from forest soil after enrichment. The strain was identified at the species level by morphology, physiological-biochemical tests and 16S rRNA gene sequencing assay. The results revealed that strain A216 was accurately identified as Burkholderia fungorum, and the ITS sequence of the species was submitted to ...

1. Liver tissue showing fatty degeneration obtained from animals poisoned with phosphorus or chloroform contains a decreased amount of esterase. 2. The serum of animals poisoned with phosphorus or chloroform has a high esterase activity. 3. The increased amount of esterase in the serum is not derived from the disintegrating liver cells as the esterase in the blood of the hepatic vein is less th...

OBJECTIVE Metabolism of aspirin (acetylsalicylic acid), commonly used in older people for the prevention of cardiovascular disease, is important to the effectiveness of this drug. Whereas part of aspirin hydrolysis occurs in blood, there is a paucity of information in regards to circulating aspirin esterase activity in various physiological and pathological conditions. High aspirin esterase act...

this study was aimed to evaluate the efficacy of dipstick tests (leukocyte esterase and nitrite) in diagnosis of spontaneous bacterial peritonitis (sbp) in cirrhotic patients. forty six children with ascites hospitalized between 2009 and 2010 in children medical center were enrolled in this study. reagent strip assays for leukocyte esterase and nitrite were performed on ascetic fluid and the re...

Proteins in the serine esterase family are widely distributed in bacterial phyla and display activity against a range of biologically produced and chemically synthesized esters. A serine esterase from the psychrophilic bacterium Pseudoalteromonas arctica with a C-terminal OsmC-like domain was recently characterized; here we report on the identification and characterization of further putative e...

The simultaneous extraction and enzymatic hydrolysis of sinapine from a mustard residue was designed for the recovery sinapic acid, high value-added phenolic acid. An initial screening allowed identification sinapoyl esterase activities in commercial cocktails (Depol 740 L, Ultraflo XL, Deltazym VR AC-100, Pectinase-PL “Amano”) mono-enzymatic solution rumen feruloyl esterase. These were not ver...

A sensitive in situ assay for the plasminogen activator of transformed cells is described; it uses the fluorogenic molecule 3',6'-bis(4-guanidinobenzoyloxy)-5-(N'-4-carboxylphenyl)thioureidospiro[isobenz ofuran-1(3H),9'-[9H]xanthen]-3-one. This fluorescein derivative is an excellent active-site titrant of the esterase activity of plasmin. When transformed cells are incubated with purified plasm...

Lactobacillus plantarum is frequently found in the fermentation of plant-derived food products, where hydroxycinnamoyl esters are abundant. L. plantarum WCFS1 cultures were unable to hydrolyze hydroxycinnamoyl esters; however, cell extracts from the strain partially hydrolyze methyl ferulate and methyl p-coumarate. In order to discover whether the protein Lp_0796 is the enzyme responsible for t...

The clinical diagnosis of Wolman's disease (acid esterase deficiency) can be confirmed by a histochemical staining method using blood films. Acid esterase activity is found normally in lymphocytes, but in Wolman's disease only a very low level of activity can be detected. An intermediate level of activity was demonstrated in heterozygotes. The method may also be applicable in the prenatal detec...