نتایج جستجو برای: in his first step

تعداد نتایج: 17114144  

Journal: :Protein science : a publication of the Protein Society 2005
Sreedevi Nallamsetty Brian P Austin Kerri J Penrose David S Waugh

Many proteins that accumulate in the form of insoluble aggregates when they are overproduced in Escherichia coli can be rendered soluble by fusing them to E. coli maltose binding protein (MBP), and this will often enable them to fold in to their biologically active conformations. Yet, although it is an excellent solubility enhancer, MBP is not a particularly good affinity tag for protein purifi...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه ارومیه - پژوهشکده ادبیات 1393

testing plays a vital role in any language teaching program. it allows teachers and stakeholders, including program administrators, parents, admissions officers and prospective employers to be assured that the learners are progressing according to an accepted standard (douglas, 2010). the problems currently facing language testers have both practical and theoretical implications but the first i...

Journal: :Applied and environmental microbiology 2005
Hyeon-Su Ro Sun Ok Jung Byung Hoon Kho Hyung Pyo Hong Jae Sung Lee Yong-Beom Shin Min Gon Kim Bong Hyun Chung

A surface plasmon resonance imaging-based Ni(2+)-iminodiacetic acid-coated gold chip system was developed to enable specific detection of a hexahistidine-tagged recombinant protein in crude extracts or in column chromatography fractions. This system is especially advantageous for high-throughput analysis of multiple proteins.

Journal: :Journal of the American Chemical Society 2008
Kalina Peneva Gueorgui Mihov Andreas Herrmann Nawid Zarrabi Michael Börsch Thomas M Duncan Klaus Müllen

Fluorescent probes are essential for the exploration of protein function, detection of molecular interactions, and conformational changes. The nitrilotriacetic acid derivatives of different chromophores were successfully used for site-selective noncovalent fluorescence labeling of histidine-tagged proteins. All of them, however, suffer from the same drawback--loss of the fluorescence upon bindi...

2013
Xinyu Zhao Guoshun Li Shufang Liang

Affinity tags have become powerful tools from basic biological research to structural and functional proteomics. They were widely used to facilitate the purification and detection of proteins of interest, as well as the separation of protein complexes. Here, we mainly discuss the benefits and drawbacks of several affinity or epitope tags frequently used, including hexahistidine tag, FLAG tag, S...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه فردوسی مشهد - دانشکده ادبیات و علوم انسانی 1391

the present dissertation aims to investigate four-word lexical bundles in applied linguistics research articles by iranian and internationally-published writers. the aims of this study are two-fold: first of all, attempts have been made to create a comprehensive list of the most commonly used four-word lexical bundles categorized by their type and token frequency, their structural characteristi...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه گیلان - دانشکده فنی 1392

due to lack of knowledge management system in the organization of technical and vocational university of iran (tvuni) and losing good employees because of retirement and substitution causes huge amount of costs to replace the similar expertise. there is no any suitable system in the tvuni to store, to document, and to distribute knowledge. based on the university’s features such as it has diffe...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه فردوسی مشهد - دانشکده الهیات و معارف اسلامی شهید مطهری 1393

murder of wife during adultery: if a man sees his wifes adultery with another man, he can kill both of them. and he is not guilty in this murder. this sentence is the opinion of imami jurists and numerous comments on this issue are the main reasons for the sentence. that is the opinion of the sunni jurists too. murder of wife and the stranger man are permitted. but in contrast to these views, ...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه رازی - دانشکده علوم 1390

in this thesis, a better reaction conditions for the synthesis of spirobarbiturates catalyzed by task-specific ionic liquid (2-hydroxy-n-(2-hydroxyethyl)-n,n-dimethylethanaminium formate), calcium hypochlorite ca(ocl)2 or n-bromosuccinimide (nbs) in the presence of water at room temperature by ultrasonic technique is provided. the design and synthesis of spirocycles is a challenging task becaus...

Journal: :bulletin of the iranian mathematical society 0
m‎. ‎hladík department of applied mathematics‎, ‎faculty of mathematics and physics‎, ‎charles university in prague‎, ‎malostranske nam‎. ‎25‎, ‎11800‎, ‎prague‎, ‎czech republic. m. černý department of econometrics‎, ‎university of economics‎, ‎n'am. w. churchilla 4‎, ‎13067‎, ‎prague‎, ‎czech republic.

‎we consider a linear programming problem in a general form and suppose that all coefficients may vary in some prescribed intervals‎. ‎contrary to classical models‎, ‎where parameters can attain any value from the interval domains independently‎, ‎we study problems with linear dependencies between the parameters‎. ‎we present a class of problems that are easily solved by reduction to the classi...

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