sperm is produced by the testis and mature in the epididymis. for having a successful conception, the fertilizing sperm should have functional competent membranes, intact acrosome, functional mitochondria and an intact haploid genome. the effects of genetic and environmental factors result in sperm vulnerability to damage in the process of spermatogenesis and maturation. in recent years, the fe...

how to cite this article: salehpour sh. diagnostic methods for neimann-pick type c. iran j child neurol. 2015 autumn;9:4(suppl.1): 16-17.   pls see pdf.

The present study was set up to formulate a generally applicable schedule for the cellular demonstration of tumour index substances using routinely prepared histological material by a method which would also be reproducible and readable and allow simultaneous histological diagnosis. The carcinoembryonic antigen (Gold and Freedman, 1965) was used as the prime subject as high-quality reagents wer...

The first stainings were tried by A VAN LEEUWENHOEK (apparently in the years 1714-1719) and by R VIEUSSENS (about 1684) studying their objects with alcoholic saffron dyes (LEWIS FT, 1942). At last, genuine histological work began much later when T HARTIG, C WEIGERT, J GERLACH, P EHRLICH and H GIERKE started in the second half of the 19 century with their systematic studies of natural and synthe...