Objective(s): We intended to determine whether the ovarian varicose which is one of the common etiologies of the pelvic congestion syndrome, has the ability to interfere with the DNA methylation reprogramming in the oocyte and thereby affect the oocyte quality or not. Materials and Methods: Varicose model was induced according to the Turner’s method in the rats. Briefly, a 20-gauge needle was p...

background: the purpose of this study was to create biomaterial scaffolds like platelet-rich plasma (prp) and platelet-rich fibrin (prf) containing stromal cell-derived factor-1 (sdf1) as a chemokine to induce hyaline cartilage regeneration of rabbit knee in a full thickness defect. methods: we created a full thickness defect in the trochlear groove of thirty-six bilateral knees of eighteen mat...

Chikungunya virus was quantitatively assayed by counting immunofluorescent foci after infection of BHK21/C13 cell monolayers. The speed and efficiency of virus attachment to cells were markedly enhanced when augmented by centrifugal force. By this procedure, a proportionality was obtained between the number of immunofluorescent foci and the volume of inoculum. Virus penetration into cells was l...

An antigen was isolated from tumor cells derived from cases of bovine lymphoma which caused the in vitro blastogenesis of lymphocytes from nine of 13 (69%) adult cattle with lymphoma. Lymphocytes derived from five of 122 (4%) normal adult cattle also underwent blastogenesis while lymphocytes from three cases of the sporadic form of lymphoma did not respond. Blastogenesis of lymphocytes from nor...

Aim. To evaluate the contribution of SOD1, CAT, PXDN1, NOS3, EDN1, VCAM, ICAM, PECAM, SELE and SELP genes to pathogenesis infective endocarditis in patients undergoing cardiac surgery. Material Methods. We studied 25 native heart valves obtained from with 13 other valvular pathology. The expression was determined by qPCR. Immunofluorescent staining performed using specific primary antibodies ni...

It was reported that M1 monoclonal antibody (M1 mab) inhibited hamster sperm-egg fusion in a dose-dependent manner. The antigen was localized to the equatorial segment (ES) of the hamster sperm, and that after the acrosome reaction, it was exposed on the surface of the sperm plasma membrane overlying the ES. The M1 antigen therefore fulfilled the main requirements of a sperm component that migh...