Rapidly adhering cells (RA cells) from the amniotic fluid of a pregnancy with fetal anencephaly were investigated by immunofluorescence assay with an antiserum against glial cells. After 24 hours' cultivation a high proportion of the cells showed positive glial-specific fluorescence, whereas no staining was seen in cells from samples of normal amniotic fluid. At the 24th week the mother was del...

Abstract Osteosarcoma is the most common primary malignant bone tumor; main treatment method surgery and adjuvant chemotherapy, with a 5-year survival rate of less than 20% for metastatic patients. Schisandrin B abundant active ingredient found in fruit Schisandra chinensis (Turcz.) Baill., Schisandraceae, which has document properties such as liver protection, antioxidant, anti-inflammatory, a...

Two anti-Aspergillus murine monoclonal antibodies (MAbs), designated 164G and 611F, have been produced; both specifically recognize cytoplasmic antigens of A. fumigatus, A. flavus, and A. niger by enzyme-linked immunosorbent assay. The MAbs can identify Aspergillus spp. both in frozen sections by immunofluorescence and in paraffin-embedded clinical specimens by immunofluorescence and immunopero...

The TRM1 gene of Saccharomyces cerevisiae encodes a tRNA modification enzyme, N2,N2-dimethylguanosine-specific tRNA methyltransferase, which modifies both mitochondrial and cytoplasmic tRNAs. The enzyme is targeted to mitochondria for the modification of mitochondrial tRNAs. Cellular fractionation and indirect immunofluorescence studies reported here demonstrate that this enzyme is also localiz...

An antiserum raised against whole cells of Thiobacillus ferrooxidans was allowed to react with a variety of acidophilic and nonacidophilic bacteria in an enzyme-linked immunosorbent assay and an indirect immunofluorescence assay. Both experiments demonstrated that the antiserum was specific at the species level. This preparation was used to evaluate the role of T. ferrooxidans in the microbial ...

Monoclonal antibodies conjugated with fluorescein permitted rapid, strain-specific identification of influenza A isolates and type-specific identification of influenza B isolates by direct immunofluorescence staining. Identification of H1 influenza A strains could be accomplished by direct immunofluorescence on cell culture fluids lacking sufficient hemagglutinin activity to permit identificati...

The diagnostic classification of glomerulonephritis is determined by the interplay of changes seen using light, immunofluorescence, and electron microscopy of the renal biopsy. Routine direct immunofluorescence on fresh tissue is currently considered the gold standard for the detection and characterization of immune deposits. We recently found a peculiar form of glomerular immune complex deposi...

Danielsson (1965) has recently described the use of non-fluorescent membrane filters and immunofluorescence staining for the identification of certain bacteria in water. The principal advantages of the procedure were that it was rapid; that it could separate and identify a small number of organisms from a relatively large volume of fluid; and that it allowed a quantitative estimate of the conce...

Cover image: immunofluorescence staining of tTG (green) and tTG activity (red) in a brain blood vessel of the APP23 Alzheimer mouse model as used in Chapter 5. APP23 mice are originally from healthcare company Novartis. Image printed with permission of Novartis. Chapter images: immunofluorescence stainings of collagen (green) in culture plates after removal of cells, as described in Chapter 4.

Formalin-fixed, Paraplast-embedded tissue sections mounted on subbed slides were digested with crude trypsin in modified Sorensen phosphate buffer containing sodium fluoride before immunofluorescence staining. This method prevented detachment of sections from slides during processing, thereby permitting immunofluorescence and histological examinations of adjacent serial sections.