We report a set of novel immunoassays in which proteins of interest can be detected using specific element-tagged antibodies. These immunoassays are directly coupled with an inductively coupled plasma mass spectrometer (ICPMS) to quantify the elemental (in this work, metal) component of the reacted tagged antibodies. It is demonstrated that these methods can detect levels of target proteins as ...

Nephelometry has become a very reliable and convenient method for immunoassays. Recent innovations in polymer chemistry have led to the design of highly sensitive and reproducible immunoassays. Key advances include the preparation and use of particles with polystyrene cores covered by thin shells with chemically reactive groups. The shells allow a covalent binding of the immunochemicals of inte...

The aim of this work is to develop a completely integrated Lab-On-Chip (LOC) for easy, rapid and cost-effective immunoassays. The pathogen sensing system is composed of a microfluidic channel surrounded by planar microcoils which are responsible for the emission and the detection of magnetic fields. The system allows the detection and quantification of superparamagnetic beads used for immunoass...

Human flavivirus group-reactive, dengue complex-reactive, and encephalitis virus complex-reactive antibodies were detected using epitope-blocking immunoassays in which the binding of selected mouse monoclonal antibodies to flavivirus antigens was blocked by human serum. When late (greater than 6 months after illness) convalescent sera were tested, the epitope-blocking immunoassays were superior...