Dr. Beatriz L. Gomez and colleagues described a new inhibition immunoassay for the diagnosis of histoplasmosis (1). My laboratory developed and extensively evaluated an antibody sandwich immunoassay for this purpose. It is important to contrast the two methods, as their performance characteristics differ. The characteristics of the assay developed in my laboratory are high sensitivity in dissem...

Topiramate is a newer anticonvulsant used to treat epilepsy, migraines, bipolar disorder, posttraumatic stress, and other conditions. Serum topiramate concentrations are measured to determine optimal levels, address therapeutic failure or drug–drug interactions, and assess compliance. Two high-throughput assays for serum topiramate measurement were compared: the Seradyn fluorescence polarizatio...

An automated fluorescence polarization immunoassay for the determination of vancomycin levels in serum was evaluated. The vancomycin assay is a homogeneous competitive inhibition immunoassay based on changes in fluorescence polarization that occur with antibody binding. This assay was compared with a liquid chromatographic assay and an agar well diffusion bioassay method by using clinical serum...

The rate-limiting step in many solid-phase immunoassays is associated with the slow kinetics of binding of macro-molecular antigen and conjugate to the immobilized phase. We demonstrate that the use of ultrasonic energy to enhance mass transport across liquid/solid interfaces can dramatically accelerate antigen binding to immobilized antibodies. We use an ultrasound-accelerated procedure with a...

A novel rolling circle amplification (RCA) immunoassay based on DNA enriching magnetic nanoparticles and assembled fluorescent DNA nanotags, magnetic nanoparticles-RCA immunoassay, is developed as a versatile fluorescence assay platform for highly sensitive proteins detection.

Abstract Background The high quality of antibody (Ab) is critical for an immunoassay; usually, Ab with low affinity often regarded as a “bad” one in the immunoassay development. How to use develop highly sensitive still huge challenge. Methods In this study, heterologous strategy was designed enhance sensitivity detection banned dye, rhodamine B (RB), fraudulent food. RB could not recognize by ...

A comparison is made of enzyme-immunoassay and radio-immunoassay for the detection of antibody in Chagas's disease, sleeping sickness, malaria, schistosomiasis ans invasive amoebiasis. Both assays were sensitive and reproductible and gave comparable results.

A novel and highly sensitive immunoassay method based on surface enhanced Raman spectroscopy (SERS) and magnetic particles has been developed. This method exhibits great potential application in bio-separation and immunoassay.

We expect and encourage narrow performance ranges within-laboratory, since it is here that the greatest control over laboratory error is possible. This is well illustrated by Gilbert (2), where he indicates that about two-thirds of the inter-laboratory error is attributable to within-laboratory performance characteristics. We thank Dr. Bogdan for his letter and are grateful for the opportunity ...

We have evaluated four current Food and Drug Administration-cleared rapid tests for human immunodeficiency virus (HIV)-specific antibodies with a panel of specimens from recently infected individuals. Recent infection was detected by RNA-based screening coupled with enzyme immunoassay-based testing. We found that the sensitivities of the various rapid tests vary greatly with regard to their abi...